| 李小平，郝朝亮，高庭，付晨星.鼠胚实验在新建胚胎培养实验室质量控制中的作用.[J].中南医学科学杂志.,2013,41(1):21-23. |
| 鼠胚实验在新建胚胎培养实验室质量控制中的作用 |
| The Role of Mouse Embryo Assay in the Quality Control ofthe Newly Established Embryonic Laboratory |
| 投稿时间：2012-09-11 |
| DOI： |
| 中文关键词: 鼠胚实验 体外受精 质量控制 |
| 英文关键词:mouse embryo assay in vitro fertilization quality control |
| 基金项目:湖南省卫生厅资助项目(B2011-046). |
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| 摘要点击次数: 833 |
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| 中文摘要: |
| 目的采用鼠胚实验（mouse embryo assay,MEA）检测新建的胚胎培养实验室的培养体系是否符合人类胚胎体外培养的要求。方法采用配子体外授精法和收集2细胞胚胎培养法进行实验。在胚胎体外培养的过程中定时对胚胎的发育状况进行观察并拍照记录。结果配子体外受精法的囊胚形成率为90.84%±7.09%。收集2细胞胚胎培养法的囊胚形成率为91.17%±6.97%。两种方法的囊胚形成率之间差异无显著性。结论配子体外授精法和收集2细胞胚胎培养法均可作为新建胚胎培养实验室的质量控制方法。 |
| 英文摘要: |
| ObjectiveTo detect whether embryo culture system of the newly established embryonic laboratory is reliabile for the human embryo culture by using mouse embryo assay.MethodsThe mouses gamete IVF and 2cell embryo collection was employed in the study.The embryo development status was observed and picture record was taken at regular time.ResultsIn in-vitro fertilization (IVF) study,the fblastocyst formation rate was 90.84%±7.09%.The blastocyst formation rate was 91.17%±6.97% in 2 cells embryos collection study.The blastocyst formation rate of the two methods was not statistically different.ConclusionThe study results show that the in-vitro fertilization (IVF) method of mouses gamete and the 2 cells embryos collection method can be used in newly established embryonic laboratory quality control. |
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