谢黎明,贺荣芳,张志伟,贺修胜.miR-124通过靶向调控DNMT3B抑制胃癌细胞增殖.[J].中南医学科学杂志.,2013,41(1):10-12. |
miR-124通过靶向调控DNMT3B抑制胃癌细胞增殖 |
MiR-124 Suppresses Cell Proliferation by TargetingDNMT3B in Gastric Carcinoma |
投稿时间:2012-11-13 |
DOI: |
中文关键词: 胃癌 miR-124 DNMT3B 细胞增殖 |
英文关键词:gastric carcinoma miR-124 DNMT3B cell proliferation |
基金项目:国家自然科学基金(31100935). |
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中文摘要: |
目的明确miR-124是否通过靶向调控DNA甲基化转移酶3B(DNA methyltransferase 3B,DNMT3B)表达而抑制胃癌细胞增殖能力,从而揭示miR-124的抑瘤分子机制。方法采用MTT检测miR-124对人胃癌MKN-45细胞的增殖能力;构建DNMT3B 3′UTR-荧光素酶报告载体,通过荧光素酶报告检测观察miR-124对DNMT3B 3′UTR-荧光素酶活性的影响;将miR-124 mimics转染胃癌细胞MKN-45,采用Western blot检测DNMT3B表达水平。 |
英文摘要: |
ObjectiveTo explicit whether miR-124 suppresses cell proliferation by targeting DNMT3B,thus to reveal molecular mechanism of miR-124 function as a tumorsuppressor in gastric carcinoma.MethodsDNMT3B 3′UTR-luciferase vector was constructed and luciferase reporter gene assay was employed to examine the effect of miR-124 on luciferase activity.MKN-45 cells were transfected with miR-124 mimics,and next Western blotting was performed to detect the expressions of DNMT3B protein.ResultsAfter transfection of miR-124 mimics for 24 h,48 h,72 h respectively,MTT assay showed that the OD values were (0.264±0.023),(0.377±0.041) and (0.524±0.029),compared with the control group (0.414±0.051),(0.619±0.065) and (0.898±0.072),the groups were significantly different (all P<0.05),and it is on a time-dependent manner.Luciferase reporter vector system confirmed that DNMT3B was a target gene of miR-124.Western blot showed that the expressions of DNMT3B protein were inhibited by miR-124.ConclusionmiR-124 suppresses cell proliferation by targeting DNMT3B in gastric carcinoma. |
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