| 李乐，唐双阳，何淑雅，孙建湘，张炜，龙望远，潘丹，欧阳赢，曹茜.IL-12对辐射损伤小鼠IFN-γ和IL-4水平的影响.[J].中南医学科学杂志.,2012,40(5):448-451. |
| IL-12对辐射损伤小鼠IFN-γ和IL-4水平的影响 |
| Effect of IL-12 on IL-4 and IFN-γ Contents of Mice Injured by Ionizing Radiation |
| 投稿时间：2012-02-24 |
| DOI： |
| 中文关键词: IL-12 辐射 免疫 |
| 英文关键词:IL-12 Radiation Immune. |
| 基金项目:湖南省大学生研究性学习和创新性实验计划项目（2010-193）. |
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| 中文摘要: |
| 目的探讨IL-12对电离辐射损伤小鼠细胞因子的调节作用，为临床免疫损伤防护提供实验依据。方法64只小鼠随机分为8组：正常对照组、单纯辐射损伤对照组、辐射损伤前+空载体对照组、辐射损伤前后+空载体对照组、辐射损伤后+空载体对照组、辐射损伤前+IL-12组、辐射损伤前后+IL-12组和辐射损伤后+IL-12组。ELISA检测脾细胞上清中IFN-γ和IL-4的含量；MTT法检测淋巴细胞增殖情况。结果辐射损伤前+IL-12组、辐射损伤前后+IL-12组和辐射损伤后+IL-12组的IFN-γ含量明显高于单纯辐射损伤对照组以及相对应的空载体对照组(P<0.05)，但脾淋巴细胞增殖情况与单纯辐射损伤对照组以及相应的空载体对照组比较差异无显著性(P>0.05)；辐射损伤前后+IL-12组和辐射损伤后+IL-12组的脾脏指数和IL-4含量明显高于单纯辐射损伤对照组以及相对应的空载体对照组(P<0.05)。结论IL-12对辐射损伤后小鼠免疫功能修复有一定影响。 |
| 英文摘要: |
| ObjectiveTo discuss the regulatory of IL-12 on cytokines of the mice injured by the ionizing radiation， so as to provide the experimental reference for the clinical protection from immune injury.MethodsA total of 64 mice were divided into 8 groups： the control group， group with only radiation injury， group injected empty vector before the ionizing radiation， group injected empty vector before/after the ionizing radiation， group injected empty vector after the ionizing radiation， group injected IL-12 vector before the ionizing radiation， group injected IL-12 before/after the ionizing radiation and group injected IL-12 vector after the ionizing radiation.The content of IFN-γ and IL-4 were detected by ELISA. The spleen lymphocytes proliferation were measured by MTT assay.ResultsThe contents of IFN-γ in the group injected IL-12 vector before the ionizing radiation， the group injected IL-12 before/after the ionizing radiation and the group injected IL-12 vector after the ionizing radiation was higher than that in the group with only radiation injury or their corresponding empty vector Group respectively (P<0.05)， but the differences of their lymphopoiesis were all non-significance (P>0.05). The spleen index and the contents of IL-4 in the group injected IL-12 before/after the ionizing radiation and in the group injected IL-12 vector after the ionizing radiation were statistically higher than these in the group with only radiation injury or their corresponding empty vector Group(P<0.05).ConclusionIL-12 can help the mice injured by the ionizing radiation to restore their immune function. |
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