| 唐双阳，范瑞岗，余敏君，刘越，张艳，朱翠明，李忠玉，万艳平.Daxx与PML在HeLa和Caski细胞株中的定位观察.[J].中南医学科学杂志.,2012,40(5):440-442. |
| Daxx与PML在HeLa和Caski细胞株中的定位观察 |
| Location of PML and Daxx in Caski Cells and HeLa Cells |
| 投稿时间：2012-06-05 |
| DOI： |
| 中文关键词: Daxx PML 宫颈癌 人乳头瘤病毒 |
| 英文关键词:Daxx PML cervical cancer human papillomavirus |
| 基金项目:国家自然科学基金面上项目(No:30973402)；湖南省教育厅重点项目(11A102). |
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| 中文摘要: |
| 目的分析宫颈癌细胞内早幼粒细胞白血病蛋白（PML）和死亡域相关蛋白（Daxx）的定位，为进一步研究高危型HPV16和HPV18的致癌机制奠定试验基础。方法培养HeLa细胞和Caski细胞，利用间接免疫荧光试验，在激光共聚焦显微镜下观察并分析PML和Daxx在细胞内的定位；分析Caski细胞中Daxx与HPV16 E6蛋白定位。结果在HeLa细胞和Caski细胞，PML红色信号和Daxx绿色信号在胞核和胞浆呈弥散样均匀分布；Daxx绿色信号与PML红色信号重叠时，依然显示各自的绿色信号与红色信号。在Caski细胞，HPV16 E6和Daxx在细胞中分布不均匀，呈区域性聚集；表达红色信号的HPV16 E6与表达绿色信号的Daxx重叠成黄色信号。结论在Caski细胞和HeLa细胞，PML定位于细胞核的PML-NBs；Daxx在胞核和胞浆呈弥散样均匀分布；两者未发生共定位。Daxx与HPV16 E6蛋白在Caski细胞共定位于胞浆与胞核。 |
| 英文摘要: |
| ObjectiveTo provide the experimental basis for further studying effects of human papillomavirus type 16（HPV16）or HPV18 on the oncogenic mechanisms，the location of Daxx and PML in the cervical cancer cells, such as Caski cells and HeLa cells, was analyzed.MethodsIndirect immunofluorescence assay was used to analyze the subcellular location of PML and Daxx in HeLa and Caski cells under confocal microscopy. It was also used to analyze the colocalization of Daxx and HPV16 E6 protein in Caski cells.ResultsThe green signals of Daxx and the red signals of PML were dispersed uniform in the nuclei and cytoplasm of HeLa cells or Caski cells. There was not the yellow signal when the green signals of Daxx and the red signals of PML were merged. The distribution of HPV16 E6 and Daxx was not uniform in Caski cells, but regional aggregation. The yellow signals were shown when the red signals of HPV16 E6 merged with the green signals of Daxx.ConclusionsThere is not any colocalization of Daxx which diffused in the nuclei and cytoplasm and PML which concentrated in PML-NBs of cytoplasm of Caski cells or HeLa cells. There are colocalizations of Daxx and HPV16 E6 protein in the nuclei and cytoplasm of Caski cells. |
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