| 周文胜，王佳，李庆华，任翔，王淼霞.阿托伐他汀对血管性痴呆大鼠脑组织Aβ和COX-2表达的影响.[J].中南医学科学杂志.,2012,40(4):345-350. |
| 阿托伐他汀对血管性痴呆大鼠脑组织Aβ和COX-2表达的影响 |
| Effect of Atorvastatin on the Expression of AΒ And COX-2 in the Brain Tissue of Rats With Vascular Dementia |
| 投稿时间：2012-04-11 |
| DOI： |
| 中文关键词: 血管性痴呆 阿托伐他汀 β淀粉样蛋白 环氧合酶-2 水迷宫实验 炎症 |
| 英文关键词:vascular dementia atorvastatin Aβ COX-2 morris water maze test inflammation |
| 基金项目:湖南省科技厅资助项目(NO. 2008FJ3195). |
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| 中文摘要: |
| 目的观察血管性痴呆（VaD）大鼠行为学改变和脑组织β-淀粉样蛋白（Aβ）、环氧合酶-2（COX-2）表达的变化，以及阿托伐他汀的干预效应，并探讨其作用机制。方法60只健康雄性SD大鼠随机分为假手术组、模型组和阿托伐他汀组（低剂量组和高剂量组）。模型组及干预组通过双侧颈总动脉结扎法建立VaD大鼠模型，术后8周通过Morris水迷宫进行行为学检测，随后免疫组织化学检测脑组织Aβ及COX-2表达水平。结果与假手术组比较，模型组大鼠定位航行试验的逃避潜伏期明显延长（P<0.05），空间探索试验的跨越平台次数减少（P<0.05），脑组织Aβ、COX-2表达上调（P<0.05）；与模型组比较，阿托伐他汀组大鼠定位航行试验的逃避潜伏期缩短（P<0.05），空间探索试验的跨越平台次数增加（P<0.05），脑组织Aβ、COX-2表达显著下降（P<0.05）；高剂量与低剂量组大鼠水迷宫成绩及脑组织Aβ、COX-2表达差异有显著性（P<0.05）。结论阿托伐他汀可能通过降低脑组织Aβ、COX-2的表达，显著改善VaD大鼠的行为学症状。 |
| 英文摘要: |
| ObjectiveTo observe the effect of atorvastatin on the expression of Aβ and COX-2 in the brain tissue of rats with vascular dementia. Methods60 healthy male SD rats were randomly divided into sham-operation group, model group, atorvastatin group(low-dose group and high-dose group). Rat vascular dementia model was prepared by permanent bilateral common carotid arteries occlusion. After 8 weeks, all rats were tested with Morris water maze, and then immunohistochemistry was used to test the expression of Aβ and COX-2 in the brain. ResultsCompared with sham-operation group, rats in model group had significantly longer escape latency in orientation navigation experiment(P<0.05), and had obviously decreased the times of crossing the platform in spatial probe test (P<0.05). The expression of Aβ and COX-2 were up-regulated(P<0.05); compared with model group, rats in atorvastatin group had significantly shortened escape latency in orientation navigation experiment(P<0.05), and had obviously increased the times of crossing the platform in spatial probe test (P<0.05). The expression of Aβ and COX-2 were down-regulated(P<0.05); the results of Morris maze test and the expression of Aβ and COX-2 between the low-dose group and the high-dose group were significantly different(P<0.05). ConclusionAtorvastatin may improve the cognition state of rats with vascular dementia by inhibiting the expression of Aβ and COX-2 in the brain. |
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