| 洪涛，邹琪婧，钟警，曹仁贤，文格波.介导油酸对小鼠c2c12细胞PGC-1α表达的影响.[J].中南医学科学杂志.,2012,40(4):331-333. |
| 介导油酸对小鼠c2c12细胞PGC-1α表达的影响 |
| Oleic Acid Induces PGC-1α Expression in Mouse c2c12 Cells Through p38 MAPK Pathway |
| 投稿时间：2012-06-05 |
| DOI： |
| 中文关键词: 过氧化物酶体增殖物激活受体γ辅激活因子1α 油酸 p38丝裂原活化蛋白激酶 c2c12细胞 |
| 英文关键词:PGC-1α oleic acid p38 MAPK c2c12 cells |
| 基金项目:国家自然科学基金（30900707/C140406）. |
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| 中文摘要: |
| 目的研究油酸（OA）对小鼠c2c12细胞中过氧化物酶体增殖物激活受体γ辅激活因子1α（PGC-1α）表达的影响，探讨p38丝裂原活化蛋白激酶（MAPK）信号通路在其中的作用。方法以不同浓度油酸及p38抑制剂SB203580（SB）分别处理c2c12细胞， 逆转录-聚合酶链反应（RT-PCR）检测PGC-1α mRNA表达， Western Blot检测PGC-1α蛋白表达。结果油酸处理组PGC-1α mRNA及蛋白表达明显高于对照组(P<0.05); OA+SB组PGC-1α表达减低(P<0.05)。结论油酸能促进c2c12细胞PGC-1α mRNA转录和蛋白的表达，该作用可能与p38 MAPK信号通路有关。 |
| 英文摘要: |
| ObjectiveTo investigate the effect of oleic acid（OA） on the expression of PGC-1α mRNA and protein in mouse c2c12 cells and to unravel the mechanism of its transcriptional regulation. MethodsCultured c2c12 cells were treated by oleic acid with or without SB203580（SB） respectively; PGC-1α mRNA was detected by RT-PCR; PGC-1α protein were detected by Western Blot. ResultsCompared with control group, the expression of PGC-1α mRNA and protein increased significantly in OA groups (P<0.05); compared with the OA group, the expression of PGC-1α mRNA and protein decreased significantly in OA+SB group (P<0.05). ConclusionOleic acid can stimulate the expression of PGC-1α mRNA and protein in c2c12 cells; oleic acid might regulate the PGC-1α gene expression through p38 MAPK pathway. |
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