| 王桥生，符晖，曾红科.线栓法大鼠局灶脑缺血模型的改进与评价.[J].中南医学科学杂志.,2012,40(3):291-294. |
| 线栓法大鼠局灶脑缺血模型的改进与评价 |
| The Improvement of Focal Cerebral Ischemia Model of Permanent Middle Cerebral Artery Occlusion Induced by Suture Method |
| 投稿时间：2011-11-22 |
| DOI： |
| 中文关键词: 线栓法 大鼠 大脑中动脉阻塞 脑缺血 |
| 英文关键词:suture method rat middle cerebral artery occlusion cerebral ischemia |
| 基金项目:广东省科技计划基金（2007B031501005）资助. |
|
| 摘要点击次数: 883 |
| 全文下载次数: 1772 |
| 中文摘要: |
| 目的评价改进后的线栓法大鼠永久性大脑中动脉阻塞局灶脑缺血模型（PMCAO）的使用价值。 |
| 英文摘要: |
| ObjectiveTo study the worth of focal cerebral ischemia model of permanent middle cerebral artery occlusion (PMCAO) induced by suture method improved from the classic method.MethodsSD rats were randomly divided into three groups:normal control group (n=8);sham-operated group (n=8);cerebral ischemia group,in which the rats were divided into 6 h or 24 h cerebral ischemia group (n=8 for each).Cerebral infarction area was assessed by staining with 2% 2,3,5-triphenyltetrazolium chloride (TTC) solution and pathological changes of cerebral infarction was assessed by staining with hematoxylin-eosin(H-E).ResultsCerebral infarction area continuously increased and pathological changes of cerebral infarction continuously exacerbated at ischemic hemisphere from 6 h to 24 h after middle cerebral artery occlusion,while similar changes did not found in normal control and sham-operated rats.In additional,to use this method,90% of them is successful.ConclusionThe modified PMCAO model can be operated simply and is reliable,stable and repeatable,and cerebral infarction is consistent with the clinical pathological process.It is perfect PMCAO model to study focal cerebral ischemic injury. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |