| 赵小燕，沈渠深，左长清，林晓，潘文嘉，戴忠.大剂量维生素C对DNA损伤及槲皮素-7-硫酸酯钠的保护作用.[J].中南医学科学杂志.,2012,40(3):248-252. |
| 大剂量维生素C对DNA损伤及槲皮素-7-硫酸酯钠的保护作用 |
| Protective Effect of Sodium Quercetin-7-mono-sulfonate on High-dose Vitamin C Induced DNA Damage |
| 投稿时间：2012-03-20 |
| DOI： |
| 中文关键词: 槲皮素-7-硫酸酯钠 槲皮素 维生素C DNA |
| 英文关键词:sodium quercetin-7-mono-sulfonate quercetin vitamin C DNA |
| 基金项目:广东省科技计划项目（No.2007B010600038）资助. |
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| 中文摘要: |
| 目的探讨大剂量维生素C（VC）对小牛胸腺DNA损伤及槲皮素-7-硫酸酯钠的保护作用。方法 |
| 英文摘要: |
| ObjectiveTo investigate the damage effect of high-dose Vitamin C(VC）on calf thymus DNA and the protective effect of sodium quercetin-7-sulfonate.MethodsCalf thymus DNA incubated with VC（0.1,0.25,0.50 mmol/L;quercetin and sodium quercetin-7-sulfonate were pretreated or co-treated with VC (0.50 mmol/L) in calf thymus DNA.The changes of DNA were analyzed by agrose gel electrophoresis and spectrophotometry.ResultsThe DNA ladder and DNA concentration had no obvious changing in 0.10 mmol/L VC group,but DNA ladder disappeared and the concentration of DNA obviously increased in 0.25 and 0.50 mmol/L VC groups.Pretreatment or co-treatment with quercetin or sodium quercetin-7-sulfonate,the DNA ladder appeared more clearly and the concentration of DNA decreased obviously.ConclusionsThese suggest that high-dose VC could induce calf thymus DNA damage and sodium quercetin-7-sulfonate has protective effect on high-dose VC induced DNA damage. |
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