| 童细焱，邵志滨，谢海龙.DADS诱导小细胞肺癌细胞株NCI-H446凋亡及其分子机制.[J].中南医学科学杂志.,2012,40(3):227-231. |
| DADS诱导小细胞肺癌细胞株NCI-H446凋亡及其分子机制 |
| DADS Induced Small-cell Lung Cancer Cell line NCI-H446 Apoptosis and its Molecular Mechanism |
| 投稿时间：2011-11-30 |
| DOI： |
| 中文关键词: DADS 小细胞肺癌 Bcl-2 凋亡 |
| 英文关键词:DADS small cell lung cancer Bcl-2 apoptosis |
| 基金项目:国家自然科学基金项目（81172576）资助. |
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| 中文摘要: |
| 目的探讨二烯丙基二硫（DADS）诱导人肺癌细胞株NCI-H446凋亡及其分子机制。方法流式细胞仪检测DADS诱导NCI-H446细胞凋亡率；激光共聚焦显微镜检测NCI-H446细胞内Ca2+水平变化；利用免疫细胞化学法检测NCI-H446细胞内Bcl-2蛋白表达。结果与对照组相比，DADS处理组细胞的凋亡率显著增加(P<0.05)；DADS处理组细胞内Ca2+水平升高(P<0.05)。DADS下调Bcl-2(P<0.05)。结论DADS诱导小细胞肺癌细胞株NCI-H446凋亡，其具体机制可能与DADS下调Bcl-2蛋白表达和促进细胞内Ca2+水平升高有关。 |
| 英文摘要: |
| ObjectiveTo explore DADS induced small-cell lung cancer cell line NCI-H446 apoptosis and its molecular mechanism.MethodsThe apoptosis rate of NCI-H446cell line induced by DADS was analyzed using flow cytometry (FCM);The change of Intracellular free calcium concentration was measured by laser scanning confocal microscope (LSCM).Immunocytochemical staining was used to detect the expression of Bcl-2.ResultsIntracellular Ca2+concentration in DADS groups was higher than the control group(P<0.05).DADS reduced BCL-2 expression (P<0.05).ConclusionsDADS could induce NCI-H446 cells apoptosis,which mechanism may be related to elevating levels of intracellular Ca2+ and decreasing the expression of Bcl-2. |
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