| 蒋艳，姜孝新，雷小勇.ghrelin抑制ox-LDL诱导的人脐静脉内皮细胞粘附分子表达.[J].中南医学科学杂志.,2012,40(2):144-146. |
| ghrelin抑制ox-LDL诱导的人脐静脉内皮细胞粘附分子表达 |
| The Inhibitory Effect of Ghrelin on ox-LDL-mediated Adhesion Molecule Expression in hUVECs |
| 投稿时间：2011-12-14 |
| DOI： |
| 中文关键词: 动脉粥样硬化 ghrelin 血管内皮细胞粘附分子-1 细胞间粘附分子-1 |
| 英文关键词:atherosclerosis ghrelin VCAM-1 ICAM-1 |
| 基金项目:湖南省教育厅课题（11C1110）. |
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| 中文摘要: |
| 目的探讨不同剂量ghrelin预处理对氧化型低密度脂蛋白（ox-LDL）诱导的人脐静脉内皮细胞粘附分子表达的作用。方法人脐静脉内皮细胞经传代培养后随机分配为对照组、ox-LDL组及1 ng/mL、10 ng/mL与100 ng/mL ghreli预处理组，采用逆转录—聚合酶链反应（RT-PCR）检测各组血管内皮细胞粘附分子-1(VCAM-l)与细胞间粘附分子-1（ICAM-1）的RNA水平变化。结果与空白对照组相比：ox-LDL组内皮细胞VCAM-1与ICAM-1分子的表达水平明显升高（P<0.05）。与ox-LDL组相比，1 ng/mL、10 ng/mL、100 ng/mL ghrelin预处理可使内皮细胞VCAM-1 mRNA含量依次降低33%、52%、63%，内皮细胞ICAM-1 mRNA分子表达水平依次降低18%、46%、57%。结论ghrelin对ox-LDL诱导的人脐静脉内皮细胞中粘附分子VCAM-1及ICAM-1的表达有明显的抑制作用。 |
| 英文摘要: |
| ObjectiveTo investigate the inhibitory effect of different concentration of ghrelin on ox-LDL-mediated adhesion molecule expression in human umbilical vein endothelial cells（hUVECs).MethodsAfter subculture,hUVECs were randomly divided into control group,ox-LDL group,ghreli-pretreated group of 1 ng/mL,10 ng/mL and 100ng/mL.RT-PCR were used to detect the RNA changes of vasculareen adhesionmolecule-1(VCAM-l) and inter-cellular adhesion molecule-1（ICAM-1).Results Compared with control group,both VCAM-1 and ICAM-1 expressions were obviously increased（P<0.05).While compared with ox-LDL group,ghrelin-pretreated groups showed a decrease in mRNA level of VCAM-1,which were 33%,52% and 63% respectively by different concentrations of 1,10 and 100 ng/mL ghrelin.The mRNA level of ICAM-1 in ghrelin-pretreated groups were also inhibited,with a decrease of 18%,46% and 57% respectively by different concentrations of 1,10 and 100 ng/mL ghrelin.ConclusionGhrelin could repress the expression of VCAM-1and ICAM-1 induced by ox-LDL in human umbilical vein endothelial cells. |
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