| 周军媚，姚峰，刘鑫，王宗保，吴端生，陈春芳，贺震，余坚.小鼠睾丸基因PDZD9的克隆及亚细胞定位分析.[J].中南医学科学杂志.,2012,40(2):127-130. |
| 小鼠睾丸基因PDZD9的克隆及亚细胞定位分析 |
| Cloning and Subcellular Location of a Novel Mouse Testis Gene PDZD9 |
| 投稿时间：2012-01-04 |
| DOI： |
| 中文关键词: PDZD9 克隆 转染 亚细胞定位 |
| 英文关键词:PDZD9 cloning transfect subcellular location |
| 基金项目:国家自然科学基金资助项目（81000035）;湖南省科技厅项目（湘财教指［2008］115）. |
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| 中文摘要: |
| 目的克隆小鼠睾丸基因PDZD9并构建pEGFP-C1-PDZD9重组载体进行亚细胞定位分析。方法 |
| 英文摘要: |
| ObjectiveTo clone a novel testis gene PDZD9，construct the eukaryotic expression vector pEGFP-C1-PDZD9 and analysis subcellular location of PDZD9 in COS-7 cell.MethodsTotal RNA was extracted from the testes of normal mature mouse，PDZD9 gene was amplified by RT-PCR and inserted into the eukaryotic expression vector pEGFP-C1.The recombinant plasmid was transfected into COS-7 cells by cationic polymer.Observation under the fluorescent microscopy was used to analysis the subcellular location of PDZD9.ResultsThe mouse gene PDZD9 was cloned and the eukaryotic expression vector pEGFP-C1-PDZD9 was constructed successfully.Observation under the fluorescent microscopy showed that PDZD9 was expressed in cytoplasm and nucleus，mainly in cell nucleus.Conclusions The gene cloning of PDZD9 was successful and initially proved the subcellular location of PDZD9，which would be benefited for further investigation on the function of PDZD9 gene. |
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