| 龙晓兰，周敏，石必枝，谢海龙，李宗海.Cofilin-1慢病毒载体的构建及其在Huh-7中的过表达.[J].中南医学科学杂志.,2012,40(1):65-69. |
| Cofilin-1慢病毒载体的构建及其在Huh-7中的过表达 |
| Construction of Lentivirus Vector Cofilin-1 and Overexpression of Cofilin-1 in Huh-7 Cells |
| 投稿时间：2011-11-30 |
| DOI： |
| 中文关键词: Cofilin-1 慢病毒载体 Huh-7细胞 |
| 英文关键词:Cofilin-1 lentiviral vector Huh-7 cells |
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| 中文摘要: |
| 目的了解衡阳市先天性唇腭裂畸形发病率及其动态变化，为制定先天性唇腭裂的预防措施提供参考依据。方法收集以医院为基础的出生缺陷监测资料，对2002～2009年衡阳市围生儿先天性唇腭裂监测资料进行分析。结果8年来衡阳市围生儿先天性唇腭裂畸形平均发病率为15.19/万,男性发病率为17.51/万,女性发病率为9.75/万,男女之比为1.79∶1,两者差异有显著性（P=0.000）；城镇发病率为9.05/万,农村发病率为17.89/万,两者差异有显著性（P=0.000）;春夏秋冬四季度发病率依次为25.32/万、9.24/万、9.63/万、19.32/万,四季度之间差异有显著性（P=0.031）；孕妇各年龄组（＜20岁，20～25岁，25～30岁，30～35岁，≥35岁）发病率分别为41.43/万、15.22/万、12.96/万、14.94/万、和48.58/万，孕妇各年龄组之间差异有显著性（P=0.000）。结论8年来衡阳市围生儿先天性唇腭裂发病率呈上升趋势；农村的先天性唇腭裂发病率明显高于城镇；冬春季节发病率高于夏秋季；孕妇年龄<20岁和≥35岁是先天性唇腭裂畸形的高发阶段。 |
| 英文摘要: |
| ObjectiveConstruct the Huh-7 cells strains which is overexpression of Cofilin-1 gene.MethodsThe complete CDS of Cofilin-1 gene was acquired by RT-PCR,and then subcloned into the lentiviral vector pWPT to construct the expression plasmid of lentiviral vector pWPT-Cofilin-1.The plasmid was identified by sequencing.Recombinant lentivirus was produced by 293 T cells following co-transfection of pWPT-Cofilin-1 or pWPT-GFP with the packaging plasmids pMD2.G and psPAX2.The recombinant lentivirus Cofilin-1 or GFP were respectively used to infect Huh-7 cells.The overexpression of Cofilin-1 in Huh-7 cells was detected by Real-Time PCR and Western blotting analysis.ResultsThe recombinant lentivirus expression plasmid pWPT-Cofilin-1 has been constructed successfully.The efficiency of recombinant lentivirus infection was greater than 95% according to the green fluorescent.The mRNA and protein of Cofilin-1 was overexpressed in Huh-7 cells.ConclusionThis study is the foundation for the further study of relationship between Cofilin-1 and hepatocellular carcinoma. |
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