| 曾钧发,肖宏,桂培根.长托宁对急性肺损伤家兔肺组织NF-κB活性的影响.[J].中南医学科学杂志.,2012,40(1):55-58. |
| 长托宁对急性肺损伤家兔肺组织NF-κB活性的影响 |
| The Effect of PHCD on the Expression of NF-κB in Acute Lung Injury Rabbits |
| 投稿时间：2011-10-18 |
| DOI： |
| 中文关键词: 急性肺损伤 内毒素 NF-κB 长托宁 |
| 英文关键词:acute lung injury polysaccharide NF-κB PHCD |
| 基金项目: |
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| 摘要点击次数: 1107 |
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| 中文摘要: |
| 目的通过研究左旋泮托拉唑钠在动物的毒性反应，为后续临床研究提供剂量设置依据。方法 |
| 英文摘要: |
| ObjectiveTo observe the effect and protective mechanism of PHCD on the expression of NF-κB in acute lung injury (ALI) rabbits induced by lipopolysaccharide (LPS).Method20 New Zealand rabbits were randomly divided into 4 groups:control group,model group,low and high PHCD-dose group.The low and high PHCD groups were intraperitoneally administrated by PHCD （0.2mg/kg and 2mg/kg,respectively） 30min before LPS injection,then the differences of lung morphology were observed.Immunohistochemistry and Western blot were used to detect the activation of NF-κB.ResultHistomorphology showed that alveolar septum became thick and infiltrated with large amount of inflammation cells,and Bronchial mucosa occasionally fell off.After the treatment of PHCD,the inflammatory response was reduced in dose-dependent manner.Immunohistochemistry showed that only a few scattered NF-κB positive cells in the bronchial epithelial and interstitial lung in the control group,while in the model group,the NF-κB -positive cells increased significantly in the mucosa,pulmonary interstitial and alveolar and vascular endothelial.After PHCD treated,NF-κB positive cells were less than the model group,and the high dose group was more obvious.Western blot showed that in the lung tissue of model group,the p65 level of NF-κB was significantly higher than the negative control group,and p65 were significantly decreased after PHCD treatment.ConclusionPHCD could effectively reduce inflammation reaction in the ALI rabbits,which may be associated with the downregulation of NF-κB activity. |
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