| 罗敏，王永胜，王嘉争，肖继，王德明.急性肺损伤大鼠肝X受体表达的变化.[J].中南医学科学杂志.,2012,40(1):47-50. |
| 急性肺损伤大鼠肝X受体表达的变化 |
| Alteration of LXR Expression in Rats Lung With Acute Injury |
| 投稿时间：2011-10-09 |
| DOI： |
| 中文关键词: 急性肺损伤 肝X受体 脂多糖 细胞因子 大鼠 |
| 英文关键词:acute lung injury liver X receptors lipopolysaccharide cytokines rat |
| 基金项目:湖南省自然科学基金（编号：11JJ3120）资助；衡阳市科技局项目（2011KJ47）资助 |
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| 中文摘要: |
| 目的探讨厄贝沙坦对心力衰竭大鼠心室电生理失稳态和L-型钙通道电流的影响。方法采用腹主动脉缩窄法建立大鼠心力衰竭模型，随机分两组，即心力衰竭治疗组与心力衰竭对照组，分别用厄贝沙坦(20 mg/kg)和安慰剂治疗，另设假手术对照组。在术后32周用测定3组大鼠电生理指标以及心功能，以急性酶解法获得单个大鼠心室肌细胞并以标准全细胞膜片钳技术记录钙通道电流。结果与假手术对照组相比，心力衰竭治疗组与心力衰竭对照组大鼠的左室舒张末压、心率和动脉血压均明显增加，而心力衰竭对照组大鼠校正QT间期和VERP明显延长，QT离散度增加，心力衰竭治疗组与心力衰竭对照组相比左心室VERP明显缩短，且心力衰竭对照组的细胞膜电容大于假手术对照组(P<0.05)，亦明显大于心力衰竭治疗组(P<0.05)，心力衰竭对照组L-型钙通道电流峰值(-926.28±334.26 pF)及电流密度(-12.61±4.55 pA/pF)稍少于假手术对照组(-921.87±468.56 pF和-13.83±7.04 pA/pF)，但差异无显著性(P>0.05)，但心力衰竭治疗组L-型钙通道电流峰值(-423.47±100.80 pF)及电流密度(-7.02±1.66 pA/pF)明显低于心力衰竭对照组和假手术对照组(P<0.05)。3组L-型钙通道电流的激活、失活和复活动力学特征差异均无显著性(P>0.05)。结论厄贝沙坦能明显改善心力衰竭大鼠电生理失稳态变化，并可能与其下调L-型钙通道电流有关。 |
| 英文摘要: |
| ObjectiveTo investigate the expression and potential role of liver X receptors（LXRs）in lung of rats with acute lung injury induced by lipopolysaccharide (LPS).Methods50 male SD rats were divided randomly into five groups:NS（normal saline）control group,LPS injured after 1 h group（LPS 1 h）,LPS injured after 2 h group（LPS 2 h）,LPS injured after 4 h group（LPS 4 h）and LPS injured after 8 h group（LPS 8 h）.Semi-quantitative reverse transcription polymerase (RT-PCR) and western-blot were used to measure expression of LXRs.The ratio of lung wet-dry weight (W/D) and arterial blood gas analysis were measured respectively.ResultsCompared to the control group,the ratio of lung wet/dry weight was significantly increased in LPS injured groups,the PaO2 was significantly decreased.The expression of LXRα/β mRNA begins to decrease at 2 h after LPS injured and significantly decreased at 4h and 8h group,While the expression of LXRα/β protein begins to decrease at 4 h after LPS injured and significantly decreased at 8h group.ConclusionThe expression of LXRs in acute lung injury (ALI) of rats induced by LPS significantly decreased.The data suggested that LXRs might involve in the regulation of inflammation in ALI. |
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