| 张彩平，韩瑛，龙石银，乔新惠，田英，丁岚，田汝芳.二苯乙烯苷对H2O2诱导损伤血管内皮.[J].中南医学科学杂志.,2012,40(1):14-16. |
| 二苯乙烯苷对H2O2诱导损伤血管内皮 |
| Effect of TSG on the Expression of MCP-1 in Vascular Endothelial Cell Induced by H2O2 |
| 投稿时间：2011-09-19 |
| DOI： |
| 中文关键词: 二苯乙烯苷 内皮细胞 动脉粥样硬化 单核细胞趋化蛋白-1 过氧化氢 |
| 英文关键词:TSG endothelial cells atherosclerosis MCP-1 H2O2 |
| 基金项目:国家自然科学基金（30800474）资助；湖南省科技厅项目（2010SK3037）资助；湖南省教育厅重点项目（11A104）资助；湖南省研究生创新项目（CX2010B385，CX2011B378）资助 |
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| 中文摘要: |
| 目的研究二苯乙烯苷（TSG）对过氧化氢（H2O2）诱导损伤的人脐静脉内皮细胞单核细胞趋化蛋白1(MCP-1)表达的影响。方法体外培养人脐静脉内皮细胞，分为空白对照组、H2O2组（200 μmol/L H2O2）、辛伐他汀组（5 μmol/L辛伐他汀+200 μmol/L H2O2）、TSG组（1 μmol/L TSG +200 μmol/L H2O2），按分组处理24 h细胞后，运用逆转录聚合酶链式反应和酶联免疫吸附试验分别检测 MCP-1 mRNA与其蛋白的表达。结果200 μmol/L的H2O2作用内皮细胞24 h后，MCP-1的mRNA和蛋白水平均较空白对照组显著升高，P<0.01。TSG预处理细胞后，MCP-1的 mRNA和蛋白水平较H2O2组降低，差异有显著性（P<0.05）。结论TSG能抑制H2O2诱导损伤的人脐静脉内皮细胞MCP-1的表达。 |
| 英文摘要: |
| ObjectiveTo study the effect of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside(TSG) on expression of monocyte chemoattractant protein-1(MCP-1) induced by H2O2 on human umbilical vein endothelial cells(HUVEC).MethodsEndothelial cells were cultured in vitro and divided into group of control,H2O2 (200 μmol/L),simvastatin (5 μmol/L simvastatin plus 200 μmol/L H2O2) and TSG (1 μmol/L TSG plus 200 μmol/L H2O2),after treated for 24 h,the expression of MCP-1 mRNA and protein were detected by reverse transcriptase polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) respectively.ResultsTreating endothelial cells with 200 μmol/L H2O2,the expression of MCP-1 mRNA and protein level was significantly higher (P<0.01).TSG can inhibit the expression of MCP-1 mRNA and protein level induced by H2O2 on endothelial cells (P<0.05).ConclusionsTSG can inhibit the expression of MCP-1 induced by H2O2 in human umbilical vein endothelial cells. |
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