| ObjectiveTo analyze the antibiotic resistance of three mucoid Escherichia coli (E.coli) from clinic.MethodsTest the drug sensitive of the three mucoid E.coli with 22 normal drugs in clinical.Use PCR technology to analyze the resistance genes such as TEM-1,IMP,OXA-23,OXA-24,AmpC,aac(3)-I,aac(6′)-I,ant(3″)-I,aph(3′),armA,rmtA,rmtB,Int-Ⅰ,Int-Ⅱ.ResultsDrug sensitivity test shows they all have produced beta-lactamase(ESBLs) which is resistant to β-lactamase drug,but have no significant resistance in common to other drugs.Resistance gene analysis shows all the experimental strains are positive in TEM-1,and negative in other resistance gene.ConclusionThree experimental strains and the common products ESBLs resistant to β-lactamase of E.coli.have no obvious difference. |