| 罗祎敏，王淑敏，张凯华.抗菌肽LL-37重组载体的构建.[J].中南医学科学杂志.,2011,39(6):643-645. |
| 抗菌肽LL-37重组载体的构建 |
| Construction of LL-37 Recombinant Vector |
| 投稿时间：2011-06-24 |
| DOI： |
| 中文关键词: 人类基因组DNA LL-37 重组载体 |
| 英文关键词:human genome DNA LL-37 recombinant vector |
| 基金项目: |
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| 摘要点击次数: 823 |
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| 中文摘要: |
| 目的从健康人的外周静脉血中获得人类基因组DNA，扩增出抗菌肽LL-37的全长基因，插入原核表达载体pQE-30以获得重组表达载体pQE-30/LL-37。方法用NaI法从健康人外周静脉血中提取人类基因组DNA，然后以它为模板PCR扩增得到LL-37全长基因，酶切回收后连接。结果得到了较高品质的人类基因组DNA，扩增得到了LL-37全长基因，成功构建了重组表达载体pQE-30//LL-37。结论用生物方法生产抗菌肽是一种很有希望的产业，而抗菌肽LL-37的重组载体的构建成功为以后进一步的实验打下了基础。 |
| 英文摘要: |
| ObjectiveTo derive human whole-genome DNA from peripheral venous blood of healthy people,to amplify the full-length genes of LL-37,to insert the prokaryotic expressing vector pQE-30 and to obtain the recombinant expression vector pQE-30/LL-37.MethodsTo derive human whole-genome DNA from the peripheral venous blood of healthy person by the means of NaI,and then the full-length genes of LL-37 will be amplified from the template of the acquired human whole-genome DNA.After the reabstraction of the restriction endonuclease from the agarose,LL-37 will be bonded with pQE-30.ResultsHigh quality human whole-genome DNA is obtained,and the recombinant vector pQE-30/LL-37 is constructed successfully.ConclusionProducing antimicrobial peptide LL-37 by the biologic method may be a promising industry,as the construction of antimicrobial peptide recombinant vector pQE-30/LL-37 can serve as a foundation to the further research. |
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