| 殷杰，杨晓燕，虞佳，向琼，朱炳阳，雷小勇.MiR-122上调野生p53蛋白增强BEL-7402/5-FU细胞对5-氟尿嘧啶敏感性.[J].中南医学科学杂志.,2011,39(6):640-642. |
| MiR-122上调野生p53蛋白增强BEL-7402/5-FU细胞对5-氟尿嘧啶敏感性 |
| MiR-122 Increases Sensitivity of BEL-7402/5-FU Cells to 5-fluorouracil via Up-regulation of Wild-type p53 Protein |
| 投稿时间：2011-06-08 |
| DOI： |
| 中文关键词: 微小RNA 野生型p53 BEL-7402/5-FU细胞 凋亡 5-氟尿嘧啶 |
| 英文关键词:miRNA wt-p53 BEL-7402/5-FU cells apoptosis 5-FU |
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| 中文摘要: |
| 目的探讨MiRNA-122对肝癌耐药细胞株BEL-7402/5-FU的5-氟尿嘧啶敏感性的影响以及作用机制。方法构建miR-122及其阴性对照空载体稳定转染至BEL-7402/5-FU细胞中，Western blot检测未转染组，阴性载体转染组和miR-122转染组中与耐药相关的p53蛋白表达水平。用MTT法和流式细胞术检测三组细胞对5-氟尿嘧啶敏感性。结果MiR-122转染组与未转染组，阴性载体转染组比较，野生型p53蛋白表达水平升高。流式细胞术检测，miR-122转染组的细胞凋亡率较未转染组和阴性载体转染组高。结论miR-122能上调野生型p53蛋白表达，增加BEL-7402/5-FU细胞对5-氟尿嘧啶敏感性,从而成为治疗肝癌耐药治疗的一个方法。 |
| 英文摘要: |
| ObjectiveTo investigate the influence and mechanism of miR-122 confer 5-FU sensitivity in BEL-7402/5-FU cells.MethodsMiR-122 and negative miRNA expression vectors were constructed and stably transfected into BEL-7402/5-FU cells.Western Blotting was used to detect Wild-type p53 （wt-p53） protein expression.Drug sensitivity of the cells to 5-fluorouracil (5-FU) were analyzed with MTT and flow cytometry.ResultsCompared with negative miRNA transfectants or untreated cells,protein expression level of wt-p53 in stable miR-122 transfectants were decreased.Flow cytometry results demonstrated that apoptosis rate increased in miR-122 transfected cells,compared with negative miRNA or untreated cells.ConclutionMiR-122 can specifically up-regulate the expression of wt-p53 in BEL-7402/5-FU cells,which increased cells spontaneous apoptosis and sensitize cells to 5-FU.Therefore,miR-122 may be used as a potential therapy agent against human hepatoblastoma. |
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