| 李跃艳，熊升林,.PKC 在S1P 激活大鼠心肌细胞核转录因子-κB 中的作用.[J].中南医学科学杂志.,2011,39(6):629-633. |
| PKC 在S1P 激活大鼠心肌细胞核转录因子-κB 中的作用 |
| Roles of PKC in S1P Induced NF-κB Activation of Cardiomyocyte Cells in Rats |
| 投稿时间：2011-08-29 |
| DOI： |
| 中文关键词: 蛋白激酶C 1-磷酸鞘氨醇 核因子κB 信号传导 |
| 英文关键词:protein knase C sphingosine-1-phosphate nuclear transcription factor -κB signal transduction |
| 基金项目: |
|
| 摘要点击次数: 810 |
| 全文下载次数: 745 |
| 中文摘要: |
| 目的探讨蛋白激酶C在1-磷酸鞘氨醇(Sphingosine 1-phosphate，S1P)激活大鼠心肌细胞核转录因子（Nuclear transcription factor，NF）-κB信号传导通路中的作用。方法心肌细胞NF-κB的核内浓度采用夹心ELISA的方法检测;免疫印记观察S1P作用前后核内NF-κB的改变；RT-PCR及免疫印记检测转化生长因子β1(transforming growth factor β1，TGF-β1)变化。结果S1P刺激可诱导心肌细胞下游NF-κB活化,两者之间存在浓度与时间的依赖关系;免疫印记显示S1P作用后,细胞核内NF-κB表达明显增加;而特异性PKC抑制剂(Staurosporine)和NF-κB阻断剂(PDTC)均能在不同程度上抑制S1P的这种作用。S1P作用心肌细胞使TGF-β1表达上调，S1P的这种作用可被Staurosporine和PDTC所抑制。结论PKC作为NF-κB活化的上游信使,参与了S1P激活NF-κB的信号传导通路。通过以上途径使TGF-β1合成增加，从而诱导心肌细胞纤维化，导致心肌肥厚。 |
| 英文摘要: |
| ObjectiveTo study the roles of protein kinase C (PKC) in the signal transduction pathway of S1P induced nuclear transcription factor (NF)-κB activation of cardiomyocyte cells.MethodNF-κB level in nuclear protein extraction of cardiomyocyte cells was detected by sandwich ELISA.Intranuclear translocation of NF-κB was observed by WB.TGF-β1protein and RNA level was detected by WB and RT-PCR.ResultS1P could induce NF-κB activation of cardiomyocyte cells in time and dose dependent manners.WB revealed intranuclear translocation following S1P induction.Specific PKC (Staurosporine) and pyrrolidine dithiocarbamate (PDTC) could inhibit S1P-induced NF-κB activation in cardiomyocyte cells.The up-expresssion of TGF-β1 by S1P was also inhibited by Staurosporine and PDTC.ConclusionPKC,as an up-stream messenger,is involved in the signal transduction pathway of S1P-induced NF-κB activation. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |