| 李跃进.酶联免疫反应加速仪在检测丙肝抗体中的应用.[J].中南医学科学杂志.,2011,39(5):579-581. |
| 酶联免疫反应加速仪在检测丙肝抗体中的应用 |
| The Roles of Enzyme-linked Immunosorbent Accelerometer Played in Detecting Antibody to Hepatitis C Virus |
| 投稿时间：2011-01-04 |
| DOI： |
| 中文关键词: 丙型肝炎 酶联免疫反应加速仪 “灰区”标本 |
| 英文关键词:HCV enzyme-linked immunosorbent accelerometer gray area |
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| 中文摘要: |
| 目的探讨酶联免疫反应加速仪在酶联免疫吸附试验(ELISA)检测丙型肝炎抗体中的应用。方法用酶联免疫反应加速仪和常规温育方法同步检测4种不同S/CO的混合血清，同时用实时荧光定量聚合酶链反应法检测临界值水平血清/灰区标本的HCV-RNA，并对结果进行比较分析。结果4种混合血清用两种方法检测的抗-HCV吸光度值（OD值）比较，差异有显著性(P<0.05)。有3种混合血清定性结果相一致，S/CO为0.9的混合血清用常规温育法检测结果为阴性，用酶联免疫反应加速仪检测结果为阳性，而检测20份S/CO为0.9的血清的HCV-RNA含量，其中14份大于1×103拷贝/mL。4种混合血清，酶联免疫反应加速仪的OD值CV%分别为2.5、2.0、2.1、2.3，常规温育方法的OD值CV%分别为3.5、4.1、4.0、4.2。结论酶联免疫反应加速仪在确定最佳反应时间条件下，具有加速抗原抗体反应的作用，可以提高ELISA检测的灵敏度，为临床判读“灰区”标本的结果提供可靠依据，避免漏诊。 |
| 英文摘要: |
| ObjectiveTo study the roles of enzyme-linked immunosorbent accelerometer played in detecting antibody to HCV by enzyme-linked immunosorbent assay (ELISA).Methods4 mixed serum of different S/CO were detected by enzyme-linked immunosorbent accelerometer and conventional incubation method,while using real-time fluorescence quantitative polymerase chain reaction assay to detect HCV-RNA of gray zone specimens.ResultsThere was significant difference (P<0.05)in anti-HCV OD values of 4 mixed serum by two methods.The qualitative results of 3 mixed serum were consistent.The result of another mixed serum which S/CO was 0.9 detected by conventional incubation method was negtive,but was positive detected by enzyme-linked immunosorbent accelerometer.Meanwhile,among 20 serum with S/CO of 0.9,HCV-RNA concentration of 14 serum was greater than 1×103 copies/mL.The CV% of OD values of 4 mixed serum by enzyme-linked immunosorbent accelerometer was 2.5、2.0、2.1、2.3,respectively,and the CV% by conventional incubation method was 3.5、4.1、4.0、4.2,respectively.ConclusionWhen the best reaction time is determined,enzyme-linked immunosorbent accelerometer can accelerate the antifen-antibody reaction,and improve the sensitivity of ELISA.So it can provide a reliable basis for the clinical interpretation of specimens in gray areas to avoid misdiagnosis. |
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