| 李波，刘良专，刘磊，罗金花，贺志国.Cpn0585蛋白的免疫活性及血清学诊断初步评价.[J].中南医学科学杂志.,2011,39(5):524-528. |
| Cpn0585蛋白的免疫活性及血清学诊断初步评价 |
| Preliminary Evaluation of Immunocompetence and Serological Diagnosis of Cpn0585 Protein |
| 投稿时间：2011-03-07 |
| DOI： |
| 中文关键词: 肺炎嗜衣原体 Cpn0585 免疫活性 血清学诊断 |
| 英文关键词:chlamydophila pneumoniae Cpn0585 immunocompetence serodiagnosis |
| 基金项目: |
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| 中文摘要: |
| 目的克隆肺炎嗜衣原体包涵体膜蛋白Cpn0585基因，初步探讨其在血清学诊断中的应用价值。 |
| 英文摘要: |
| ObjectiveTo clone and express the Cpn0585 gene from Chlamydophila pneumonia e.and then evaluate the clinical value of the recombinant protein in serodiagnosis.MethodsThe pGEX-6p-2／Cpn0585 recombinant plasmid was contructed，and then expression of recombinant protein was induced.The expressed recombinant protein was identified by SDS-PAGE.BALB／c rats were immunized with the purified protein;indirect ELISA was applied to measuring its immunogenicity；and its immunoreactivity was analyzed by reacting with Cpn-specific IgG antibody in sera.Cpn IgG and Chlamydozoa trachomatis positive sera were detected with indirect ELISA.ResultsThe recombinant protein with relative molecular weight about 95 kDa was expressed and purified effectively.Western blot analysis proved that the recombinant protein could react with human anti Cpn IgG specifically.The titer of the specific IgG antibodies in the immuned BALB／c rats was above 1∶12 800.The compliance rate between the indirect ELISA test and SeroCPTM IgG ELISA kits to104 patients sera were 98.3%.ConclusionThe expressed recombinant protein of Cpn0585 from Cpn shows excellent irnmunoc-ompetence.It is suitable for preparation of serodiagnostic EIISA reagent for Cpn infection. |
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