| 罗迪贤，李波平，高治平.TOFA协同柔红霉素诱导肠癌细胞株HCT-8细胞凋亡.[J].中南医学科学杂志.,2011,39(5):514-517. |
| TOFA协同柔红霉素诱导肠癌细胞株HCT-8细胞凋亡 |
| Combination Application of TOFA and Daunorubisin Induces Apoptosis of Colon Cancer Cell Line HCT-8 |
| 投稿时间：2010-11-22 |
| DOI： |
| 中文关键词: 结肠癌 TOFA 乙酰辅酶A羧化酶 柔红霉素 细胞凋亡 |
| 英文关键词:colon cancer TOFA acetyl-CoA carboxylase daunorubisin cell apoptosis |
| 基金项目:国家自然科学基金（30971170），湖南省自然科学基金项目（06JJ5055）. |
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| 中文摘要: |
| 目的研究TOFA及柔红霉素（DNR）联合应用对肠癌细胞株HCT-8的作用，探讨其联合应用于临床的可能性。方法将1×104个HCT-8细胞种植于96孔板，采用MTT比色法测定不同浓度TOFA或/和DNR对同步化处理后的HCT-8 细胞的生长抑制效果； DNA降解分析法（DNA Fragmentation）检测TOFA与DNR联合使用对HCT-8细胞DNA的损伤。Western blot检测TOFA与DNR联合使用对凋亡蛋白p53以及PARP的水解作用。结果 |
| 英文摘要: |
| ObjectiveTo investigate the effect of combination application of TOFA and Daunorubsin (DNR) on cell growth of colon cancer cell line HCT-8.Methods1×104 cells of HCT-8 each well were spread in 96-well plate and cultured overnight.The cells were treated with serum-free RPMI-1640 medium for 24 h for cell synchronization,and then with different concentrations of TOFA or DNR for 72 h.Finally，viable cells were detected by MTT assay.After HCT-8 cells were treated with 20 g/mL TOFA and 0.6 μmol/L DNR for 24 h,DNA was extracted from HCT-8 cells and subjected to DNA fragmentation,and protein was extracted from HCT-8 and subjected to western blot for detecting p53 and PARP.ResultsTOFA or DNR inhibited the growth of HCT-8 in a dose-dependent manner.TOFA and DNR are cytotoxic to HCT-8 cells with an IC50 at approximately 7.5 μg/mL or 0.18μmol/L,respectively.When TOFA and DNR with the concentrations of 20 μg/mL or 0.6 μmol/L were associately applied,the cell viability was only 10.4%±3.0%，contrast to control without drug treatment.The combination application of the two drugs significantly increased DNA fragmentation,and also induced the expression of p53,and the cleavage of PARP.ConclusionThe combination applicaiton of TOFA and DNR inhibits the growth of colon cancer cell line HCT-8 by inducing cell apoptosis. |
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