| 莫中成，陈欣，尹小波，曾颖，张青海，易光辉，唐朝克.PPARγ反义寡核苷酸对THP-1巨噬细胞CD36表达的影响.[J].中南医学科学杂志.,2011,39(5):493-496. |
| PPARγ反义寡核苷酸对THP-1巨噬细胞CD36表达的影响 |
| Effect of Antisense PPARγ Oligonucleotide on THP-1 Macrophage CD36 Expression |
| 投稿时间：2011-04-13 |
| DOI： |
| 中文关键词: 过氧化体增殖物激活型受体γ CD36 反义寡核苷酸 动脉粥样硬化 |
| 英文关键词:PPARγ CD36 antisense PPARγ oligonucleotide atherosclerosis |
| 基金项目:湖南省教育厅科研项目（10C1165）. |
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| 中文摘要: |
| 目的观察过氧化体增殖物激活型受体γ（PPARγ）反义寡核苷酸对THP-1巨噬细胞CD36表达及细胞胆固醇流入的影响，探讨PPARγ的作用及机制。方法用50 mg/L氧化型低密度脂蛋白（ox-LDL）分别与不同浓度PPARγ的反义寡核苷酸(400 nmol/L)、错义寡核苷酸(400 nmol/L)或15 μmol/L PPARγ激动剂ciglitazone共同孵育THP-1巨噬细胞24 h后，采用逆转录—聚合酶链反应和Western印迹分析分别检测CD36的表达，采用液体闪烁计数法检测［3H］胆固醇流入。结果与ox-LDL组比较，PPARγ反义寡核苷酸下调巨噬细胞CD36的表达，且细胞胆固醇流入率下降（ox-LDL组为28.1%、反义寡核苷酸组为22.5%）。而ciglitazone则使巨噬细胞CD36的表达上调，胆固醇流入率明显增加（36.8%）。结论抑制PPARγ表达，可抑制THP-1巨噬细胞CD36的表达，并减少细胞胆固醇流入。 |
| 英文摘要: |
| ObjectiveTo investigate the effect of peroxisome proliferator-activated receptor-γ（PPARγ） on CD36 expression and cholesterol influx in THP-1 macrophage.MethodsAfter exposure of the cultured THP-1 macrophage to antisense PPARγ oligonucleotide and ciglitazone for 24 hours,［3H］labeled Cholesterol influx was determined by FJ-2107P type liquid scintillator.CD36 mRNA and protein level were determined by reverse transcription-polymerase chain reaction(RT-PCR) and Western blotting respectively.ResultsAntisense PPARγ oligonucleotide inhibited CD36 expression and cholesterol influx in THP-1 macrophage.PPARγ agonist,ciglitazone,elevated CD36 in both protein and mRNA levels,and increased cholesterol influx in THP-1 macrophage.ConclusionOur results indicated that PPARγ may play an important role in CD36 expression and cholesterol influx in THP-1 macrophage. |
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