| 唐海林，邓敏，廖前进，曾希，周秀田，苏琦.结肠癌组织中miR-185的表达及其临床意义.[J].中南医学科学杂志.,2011,39(5):491-492. |
| 结肠癌组织中miR-185的表达及其临床意义 |
| Expression and Clinical Significance of miR-185 in Tissues of Colon Carcinoma |
| 投稿时间：2011-01-07 |
| DOI： |
| 中文关键词: 微小RNA 结肠癌 临床意义 |
| 英文关键词:microRNA-185 colon carcinoma clinical significance |
| 基金项目:国家自然科学基金（31100935 ），南华大学研究生教改课题（2009 YJS19）. |
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| 中文摘要: |
| 目的探讨miR-185（Homo sapiens miR-185，miR-185）在结肠癌组织中的表达及其临床意义。方法运用原位杂交技术（hybridization in situ，ISH）检测miR-185在结肠癌及癌旁组织中的表达情况，分析其表达与结肠癌临床分期及淋巴结转移间的关系。结果ISH检测显示，在52例结肠癌组织中miR-185阳性表达率为13.46%，与癌旁对照组（84.62%）相比，差异有显著性(P<0.01)；miR-185在结肠癌临床Ⅲ/Ⅳ期、Ⅰ/Ⅱ期和癌旁对照组中的光密度值分别为0.12±0.01、0.37±0.04、0.72±0.09，临床Ⅲ/Ⅳ期、Ⅰ/Ⅱ期分别与癌旁对照组比较，差异有显著性(P<0.05)；淋巴结转移的与无淋巴结转移miR-185表达的光密度值分别为0.11±0.02、0.39±0.04，有淋巴结转移的miR-185表达水平低于无淋巴结转移，两者差异有显著性(P<0.01)。miR-185的表达随着结肠癌临床分期演进而降低。结论miR-185与结肠癌的发生、临床进展、转移密切相关，其可能成为结肠癌治疗、预后判断的潜在生物学指标。 |
| 英文摘要: |
| ObjectiveTo investigate the expression of the miR-185 in tissue of colon carcinoma and its clinical significance.MethodsThe tissue specimens and paracancerous tissues of 52 cases were collected and compared.Hybridization in situ was used to detect the miR-185 expression in those specimens.ResultsThe positive expression rate of the miR-185 was 13.46% in colon carcinoma which was lower than the control group (84.62%)(P<0.01).It suggested that the miR-185 expression was associated with advanced clinical stage and with lymph node metastasis,stage Ⅲ/Ⅳ were higher than Ⅰ/Ⅱ tumor(P<0.05),tumors with lymph node metastasis were lower than tumors without lymph node metastasis in colon carcinoma(P<0.01).ConclusionsMiR-185 may play an important role in the proliferation,and metastasis of colon carcinoma,and it could be an indicator in the diagnosis and prognosis of colon carcinoma,miR-185 can be used as a predictive biomarker. |
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