| 董晓，何淑雅，李斌元，王三虎，自映萍，马云.FTH1真核表达载体的构建与鉴定.[J].中南医学科学杂志.,2011,39(5):487-490. |
| FTH1真核表达载体的构建与鉴定 |
| Construction and Identification of FTH1 Gene Eukaryotic Expression Vector |
| 投稿时间：2011-05-11 |
| DOI： |
| 中文关键词: 脆性X综合征 FTH1 真核表达载体 |
| 英文关键词:fragile X syndrome FTH1 eukaryotic expression vector |
| 基金项目:国家自然科学基金项目（30370795）和湖南省自然科学基金（07JJ3030）资助项目. |
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| 中文摘要: |
| 目的构建携带人铁蛋白重链多肽1（FTH1）基因的真核表达载体pCMV-Myc-FTH1，为研究FXR1P与FTH1体内相互作用奠定基础。方法以人胎脑cDNA文库为模板进行聚合酶链反应（PCR）特异扩增FTH1基因片段，将扩增片段经EcoR Ⅰ和Xho Ⅰ双酶切后克隆到同样经EcoR Ⅰ和Xho Ⅰ双酶切的pCMV-Myc载体，酶切鉴定阳性克隆并进一步测序鉴定。结果成功构建了pCMV-Myc-FTH1重组真核表达载体。结论pCMV-Myc-FTH1重组真核表达载体的构建为进一步在细胞内鉴定FXR1P与FTH1相互作用奠定了基础，对研究FXR1在脆性X综合征中的作用机理提供了依据。 |
| 英文摘要: |
| ObjectiveTo construct a eukaryotic expression vector pCMV-Myc-FTH1 that can express the human ferritin heavy peptide 1 (FTH1) in order to study the interaction between FXR1P and FTH1.MethodsThe FTH1 gene was amplified from a human fetal brain pGADT7-cDNA library plasmid,digested by EcoR I and Xho I and then cloned into eukaryotic expression vector pCMV-Myc which was also digested by EcoR I and Xho I.The recombinant plasmid of pCMV-Myc-FTH1 was identified by enzyme digestion and sequence analysis.ResultThe recombinant eukaryotic expression vector pCMV-Myc-FTH1 was successfully constructed.ConclusionThe construction of recombinant eukaryotic expression vector pCMV-Myc-FTH1 will lay the foundation further for the identification of the interaction between FXR1P and FTH1,and provide some basis for the study of the mechanism of FXR1 within fragile X syndrome. |
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