| 向琼，唐惠芳，殷杰，虞佳，杨晓燕，朱炳阳，雷小勇.MicroRNA-98对A549/DDP细胞顺铂耐药性的影响.[J].中南医学科学杂志.,2011,39(3):262-265. |
| MicroRNA-98对A549/DDP细胞顺铂耐药性的影响 |
| Effects of MicroRNA-98 on Cisplatin Resistance in A549/DDP Cells |
| 投稿时间：2011-03-31 |
| DOI： |
| 中文关键词: MicroRNA-98 A549/DDP细胞 顺铂 耐药性 |
| 英文关键词:microRNA-98 A549/DDP cells cisplatin drug resistance |
| 基金项目:国家自然科学基金资助（30900625）；湖南省教育厅资助科研项目（08A059）. |
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| 中文摘要: |
| 目的探讨MicroRNA-98（miR-98）对肺腺癌耐顺铂细胞系A549/DDP顺铂耐药性的影响。方法利用MicroRNA（miRNA）芯片及生物信息学筛选出miR-98。将miR-98质粒载体瞬时转染至A549/DDP细胞；MTT检测细胞药物敏感性的改变。结果在A549/DDP细胞中miR-98表达水平下调。转染miR-98的A549/DDP细胞生长抑制率明显增高。结论miR-98能够增加肺腺癌耐药细胞株A549/DDP对顺铂的药物敏感性。 |
| 英文摘要: |
| ObjectiveThis study investigated the effect of microRNA-98 on cisplatin resistance in cisplatin-resistant human lung adenocarcinoma cell line A549/DDP.MethodMicroRNA-98 was selected using MicroRNA microarray and bioinformatics.Pre-miR-98(TM) (miR-98) was transient transfected into A549/DDP cells.Drug sensitivity of the cells to cisplatin (DDP) was analyzed with MTT.ResultThe expression of MicroRNA-98 was marked downregulation in the A549/DDP compared to parental A549 cells.MTT results showed that miR-98 transfectants had a higher cell inhibition rate than A549/DDP cells or negative.ConclusionMicroRNA-98 increased cell spontaneous sensitized 549/DDP cells to DDP. |
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