| 王智，何湘珍，肖启国.苦参碱对兔晶状体上皮细胞内胶原合成的影响.[J].中南医学科学杂志.,2011,39(2):165-167. |
| 苦参碱对兔晶状体上皮细胞内胶原合成的影响 |
| Effects of Matrine on Collagen Synthesis of Cultured Rabbit Lens Epithelial Cells in Vitro |
| 投稿时间：2010-12-07 |
| DOI： |
| 中文关键词: 苦参碱 晶状体上皮细胞 胶原蛋白 后囊膜混浊 兔 |
| 英文关键词:Matrine lens epithelial cell collagen posterior capsular opacification rabbit |
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| 中文摘要: |
| 目的探讨苦参碱(Mat)对体外培养的兔晶状体上皮细胞(RLECs)内Ⅰ型和Ⅲ型胶原蛋白合成的影响。方法RLECs培养后分为3组：空白对照组以DMEM为培养基；重组人表皮生长因子(rhEGF)组以DMEM+50 μg/L rhEGF为培养基；Mat组以含50 μg/L rhEGF和1.0 g/L Mat的DMEM为培养基。利用50 μg/L rhEGF诱导RLECs增殖，加入1.0 g/L Mat培养24 h后，采用酶联免疫吸附测定法(ELISA)检测RLECs内Ⅰ型和Ⅲ型胶原蛋白表达。结果50 μg/L rhEGF作用后，RLECs内Ⅰ型和Ⅲ型胶原蛋白浓度分别为57.65±4.54 μg/L和1.59±0.19 μg/L，较空白对照组明显升高(P<0.01)。Mat作用后，RLECs内Ⅰ型和Ⅲ型胶原蛋白浓度分别为21.74±3.96 μg/L和0.56±0.21 μg/L，较rhbFGF组和空白对照组明显下降(P<0.01)。结论Mat能抑制体外培养的RLECs内Ⅰ型和Ⅲ型胶原蛋白的合成。 |
| 英文摘要: |
| ObjectiveTo study the effects of Matrine(Mat)on collagen synthesis of rabbit lens epithelial cells(RLECs)in vitro.MethodsCultured RLECs were divided into three groups:control group was added DMEM,rhEGF group was added DMEM and 50 μg/L rhEGF,Mat group was added DMEM,50 μg/L rhEGF and 1.0 g/L Mat.Using 50 μg/L rhEGF to induce proliferation of RLECs.Proliferative RLECs were incubated with 1.0 g/L Mat for 24 hours.The contents of collagen Ⅰ and Ⅲ of RLECs were assayed by enzyme-linked immunosorbent assay(ELISA).ResultsThe contents of collagen Ⅰ and Ⅲ of RLECs in rhEGF group were 57.65±4.54 μg/L and 1.59±0.19 μg/L respectively,which increased obviously compared with control group(P<0.01).The contents of collagen Ⅰ and Ⅲ of RLECs in1.0 g/L Mat group were 21.74±3.96 μg/L and 0.56±0.21 μg/L respectively,which decreased obviously compared with control group and rhEGF group(P<0.01).ConclusionMat could effectively inhibit the sythesis of collagen Ⅰ and Ⅲ of RLECs in vitro. |
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