| 周孝钱，许俊，唐江琼，秦旭平.CGRP对氧化损伤人脐静脉内皮细胞的保护作用及Caveolin-1表达的影响.[J].中南医学科学杂志.,2011,39(2):131-134. |
| CGRP对氧化损伤人脐静脉内皮细胞的保护作用及Caveolin-1表达的影响 |
| Effect of CGRP on Protection of Human Umbilical Vein Endothelial Cells and Caveolin-1 Expression Induced by H2O2 |
| 投稿时间：2010-11-24 |
| DOI： |
| 中文关键词: 过氧化氢 CGRP Caveolin-1 内皮细胞 动脉粥样硬化 |
| 英文关键词:H2O2 CGRP Caveolin-1 hUVECs atherosclerosis |
| 基金项目:国家自然科学基金资助（30572192）及南华大学学术带头人培养对象资金资助. |
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| 中文摘要: |
| 目的探讨降钙素基因相关肽（CGRP）对过氧化氢(H2O2)诱导离体培养的人脐静脉内皮细胞（hUVECs）损伤的保护作用及其机制。方法CGRP预孵育hUVECs后，用不同浓度H2O2处理hUVECs，噻唑蓝比色法（MTT）观察hUVECs活性；Western-blot检测Caveolin-1表达。结果CGRP（0.1～100.0 nmol/L）能呈浓度依赖性提高hUVECs活性。H2O2（0.2～5.0 mmol/L）能呈浓度依赖性降低hUVECs活性。CGRP（10～100 nmol/L）预孵育hUVECs 30 min能明显提高H2O2诱导的hUVECs抗损伤能力（P﹤0.05）；与0.1%FBS组比较，100 nmol/L CGRP和0.05 mmol/L H2O2处理组细胞Caveolin-1蛋白水平表达下降（P﹤0.05）；0.8 mmol/L H2O2处理组细胞Caveolin-1蛋白表达水平上升（P﹤0.05）；与H2O2处理组比较，100 nmol/L CGRP预孵育hUVECs 30 min能显著降低细胞Caveolin-1蛋白水平的表达（P﹤0.05）。结论CGRP对H2O2诱导的hUVECs损伤有一定的保护作用,其机制可能与下调氧化应激导致的hUVECs Caveolin-1的表达有关。 |
| 英文摘要: |
| ObjectiveTo study the protective effect of cacitonin gene-related peptide (CGRP) on human umbilical vein endothelial cells (hUVECs) injury induced by H2O2 and the related mechanism of action.MethodsCGRP and/or H2O2 were used for treatment of hUVECs.Cell viability was determined by methyl thiazolyltetrazolium (MTT) assay.Expression of Caveolin-1 was measured by Western-blot.ResultsCGRP（0.1～100.0 nmol/L）could dose-dependently induce the cell viability of hUVECs.H2O2（0.2～5.0 mmol/L）could dose-dependently impaire the cell viability of hUVECs.Pretreatment of hUVECs 30min with CGRP（10～100 nmol/L）dose-dependently increased the cell viability impaired by H2O2.Compared with 0.1%FBS group,100 nmol/L CGRP and 0.05 mol/L H2O2 down-regulated the expression of Caveolin-1,which was up-regulation of incubation of 0.8 mmol/L of H2O2.Compared with H2O2 group,the expression of Caveolin-1 was significantly decreased by pretreatment with CGRP.ConclusionCGRP has a protective effect on the hUVECs injured by H2O2;the mechanism may be related to decreasing the expression of Caveolin-1. |
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