| 阳波，李湘斌.RP-HPLC法测定人血清中地高辛浓度.[J].中南医学科学杂志.,2011,39(1):114-116. |
| RP-HPLC法测定人血清中地高辛浓度 |
| Determination of Digoxin in Serum by RP-HPLC |
| 投稿时间：2010-08-30 |
| DOI： |
| 中文关键词: 反相高效液相色谱法 地高辛 血清浓度 |
| 英文关键词:RP-HPLC digoxin the consistence of serum |
| 基金项目: |
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| 中文摘要: |
| 目的建立测定人血清中地高辛浓度测定的方法。方法将血清酸化，用丙醇∶氯仿(1∶5)萃取2次，吹干萃取液，残渣用流动相溶解，采用高效液相色谱法测定，色谱柱:Hypersil ODS C18柱(250 mm×4.0 mm，5 μm)，流动相：甲醇∶水（60∶40），检测波长：220 nm。结果地高辛浓度在0.35～2.35ng/mL范围内，地高辛和内标的峰面积比与浓度呈现良好线性关系（r=0.995 8），平均回收率103.2%～106.4%，日内和日间精密度RSD<7.5%（n=5）。结论该方法快速、简便、准确，适合于临床血药浓度监测。 |
| 英文摘要: |
| Objective To establish a method for the determination of Digoxin in serum.MethodsThe samples were acidified at first，then extracted twice by propyl alcohol- chloroform（5∶1），and the extraction were blew to dry，the leavings were dissolved by the mobile phase at last.With determination by RP-HPLC，analytical column was Hypersil ODS C18(250×4.0 mm，5 μm)，the mobile phase was consisted of methanol-water（60∶40），detected at wavelength 220 nm.ResultsThe linear rang of Digoxin was 0.35～2.35ng/mL.The mean relative recovery arranged from 103.2% to 106.4%.The relative standard deviation of within day and between day were all less than 7.5%（n=5）.ConclusionThis method is found to be rapid，accurate，sensitive and more suitable for clinical TDM. |
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