| 黄靓，张弛，谭立志，高勇强，王汉群，曾谷清.HSV-1早早期蛋白0对巨噬细胞凋亡及分泌活性的影响.[J].中南医学科学杂志.,2011,39(1):52-54. |
| HSV-1早早期蛋白0对巨噬细胞凋亡及分泌活性的影响 |
| Effect of Expression of HSV-1 Immediately Early Protein 0 on Apoptosis and Secretion of Macrophages |
| 投稿时间：2010-10-08 |
| DOI： |
| 中文关键词: ICP0 巨噬细胞 凋亡 TNF-α IL-6 |
| 英文关键词:ICP0 macrophage apoptosis TNF-α IL-6 |
| 基金项目:湖南省教育厅资助项目(02C391). |
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| 中文摘要: |
| 目的研究人单纯疱疹病毒Ⅰ型(HSV-1)早早期蛋白0(ICP0)对巨噬细胞（MΦ）凋亡及分泌活性的影响，为进一步研究ICP0蛋白在HSV-1致病机制中的作用奠定实验基础。方法将真核表达载体PT7-110转染MΦ，以抗ICP0抗体为一抗作Western blot检测其表达。在转染48 h后以ELISA法分析各组培养基中肿瘤坏死因子α（TNF-α）和白细胞介素6（IL-6）产生水平，并收集MΦ经流式细胞术(FCM)检测细胞凋亡。结果ICP0能够刺激MΦ产生TNF-α和IL-6，且能诱导MΦ发生凋亡。结论HSV-1 ICP0高表达可诱导MΦ发生凋亡并上调其分泌细胞因子TNF-α和IL-6。 |
| 英文摘要: |
| ObjectiveTo study the efect of the expression of immediately early protein 0（ICP0）on apoptosis and secretion of macrophages，and to further explore the contribution of ICP0 gene to the infection of HSV-1.MethodsMacrophages were transfected with eukaryotic expression vectors PT7-110 and the expression of ICP0 was identified by Western blotting.Macrophages were stimulated with ICP0 to analyze the production of TNF-αand IL-6.The macrophages apoptosis rate was tested by flow cytometry.ResultICP0 stimulated macrophages to produce TNF-α and IL-6 and macrophages apoptosis are also up-regulated in response to ICP0 stimulation.ConclusionThe overexpression of ICP0 may induce the apoptosis macrophages and upregulate TNF-α and IL-6 secretion of macrophages． |
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