| 曾凡香，唐爱琼，刘杰，谢宛玉.新木脂素VB-1对人卵巢癌CoC1细胞生长的影响.[J].中南医学科学杂志.,2011,39(1):33-36. |
| 新木脂素VB-1对人卵巢癌CoC1细胞生长的影响 |
| Effects of Neolignan VB-1 on the Growth of Human Ovarian Cancer CoC1 Cells |
| 投稿时间：2010-11-15 |
| DOI： |
| 中文关键词: 卵巢癌 木脂素 CDK2 CDK4 p21WAF1/CIP1 |
| 英文关键词:ovarian cancer lignan CDK2 CDK4 p21WAF1/CIP1 |
| 基金项目: |
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| 中文摘要: |
| 目的研究新木脂素 (VB-1)对人卵巢癌CoC1细胞生长的影响。方法MTT还原法测定细胞活性；琼脂培养集落形成法检测细胞集落形成能力；碘化丙啶(PI)染色、流式细胞术分析细胞周期；Western Blotting法分析CDK2、CDK4和p21WAF1/CIP1蛋白表达。结果VB-1显著抑制CoC1细胞活性(P<0.01),呈浓度依赖性,VB-1以浓度依赖方式抑制CoC1细胞集落形成能力(P<0.01),VB-1阻滞CoC1细胞周期于G1期(P<0.05)。VB-1下调CDK2和CDK4蛋白表达(P<0.05)，同时上调p21WAF1/CIP1蛋白表达P<0.05)。结论VB-1具有抑制CoC1细胞生长作用，其作用机制可能与阻滞细胞周期进展相关。 |
| 英文摘要: |
| ObjectiveTo investigate whether (VB-1)，a neolignan，inhibits the growth of human ovarian cancer CoC1 cells.MethodsCell viability was examined using MTT assay.Colony formation in CoC1 cells was detected using colony formation assay in soft agar.Cell cycle was analyzed by flow cytometry(FCM) using PI fluorescence staining.The expression of CDK2，CDK4 and p21WAF1/CIP1 protein was analyzed by Western Blot.ResultsVB-1 significantly inhibited the cell viability in CoC1 cells(P<0.01)，in a concentration-dependent manner.VB-1 reduced the colony formation in CoC1 cells(P<0.05).FCM analysis after PI stainning indicated that the percentage of G1 cell population in CoC1 cells was increased by treatment with VB-1(P<0.05).VB-1 obviously decreased the expression of CDK2 and CDK4 protein(P<0.05)，and upregulated the expression of p21WAF1/CIP1 protein(P<0.05).ConclusionVB-1 has the inhibitory effects on growth of human ovarian cancer CoC1 cell line，and its mechanisms are involved in arresting the progression of cell cycle. |
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