| 曾超，吴和平，舒筱灿，王蕾，舒旭，伍世挥.RhoC-shRNA对结肠癌细胞HT-29细胞生物学行为的影响.[J].中南医学科学杂志.,2011,39(1):25-28. |
| RhoC-shRNA对结肠癌细胞HT-29细胞生物学行为的影响 |
| The Effects of RhoC-shRNA Expression on the Biological Behavior of HT-29 Cells |
| 投稿时间：2010-11-20 |
| DOI： |
| 中文关键词: shRNA RhoC 结肠癌 侵袭 HT-29细胞 |
| 英文关键词:shRNA RhoC colon cancer invasion HT-29 cells |
| 基金项目:怀化市科技创新型人才培养计划项目资助（2007011810）. |
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| 中文摘要: |
| 目的探讨RhoC-shRNA对结肠癌细胞生物学行为的影响。方法构建RhoC-shRNA表达载体,以脂质体转染法将之稳定转染入HT-29结肠癌细胞株,Western blot检测转染细胞中RhoC蛋白表达的抑制情况,观察转染前后结肠癌细胞周期、凋亡和侵袭能力的变化。结果成功构建RhoC-shRNA真核表达载体,并且转染入结肠癌细胞后,细胞中RhoC蛋白表达水平明显受到抑制，并且抑制细胞增殖、促进细胞凋亡，降低肿瘤细胞侵袭能力。结论下调RhoC蛋白的表达可抑制结肠癌细胞HT-29的体外增殖和侵袭能力。 |
| 英文摘要: |
| ObjectiveTo investigate the effects of shRNA inhibition of RhoC expression on the biological behavior of HT-29 cells.MethodsRecombinant expression plasmid pSihRhoC targeting human RhoC gene was constructed.HT-29 cells were transfected with pSihRhoC.The cell cycle,invasiveness and apoptosis of human colon carcinoma cell line HT-29 were determined by flow cytometry,matrigel invasion assays and Hoechst 33258.ResultsRecombinant plasmid pSihRhoC was constructed successfully.The expression levels of RhoC protein were down-regulated in HT-29 transfected with pSihRhoC,and inhibited cell proliferation,promoted apoptosis and reduced tumor cell invasion.ConclusionThe Recombinant plasmid RhoC-shRNA inhibited the HT-29 colon cancer cell proliferation and invasion in vitro.Therefore,our study provided a novel way for treatment of colon cancer. |
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