| 张弛，黄靓，佘美华，尹卫东.褪黑素对ox-LDL所致巨噬细胞TNF-α释放及核因子κB活性的影响.[J].中南医学科学杂志.,2011,39(1):14-17. |
| 褪黑素对ox-LDL所致巨噬细胞TNF-α释放及核因子κB活性的影响 |
| The Effect of Melatonin on the Release of TNF-α and the Activity of NF-κB Elicited by ox-LDL |
| 投稿时间：2010-10-08 |
| DOI： |
| 中文关键词: 褪黑素 氧化型低密度脂蛋白 巨噬细胞 肿瘤坏死因子α 核因子κB |
| 英文关键词:Melatonin ox-LDL macrophage TNF-α NF-κB |
| 基金项目: |
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| 中文摘要: |
| 目的探讨褪黑素对氧化型低密度脂蛋白（ox-LDL）所致的巨噬细胞肿瘤坏死因子α(TNF-α)释放及核因子κB活性的影响。方法以ox-LDL、ox-LDL加不同浓度的褪黑素处理RAW264.7小鼠巨噬细胞，采用酶联免疫吸附测定细胞上清液中TNF-α的浓度，免疫印迹法检测p65／核因子κB的核移位，凝胶电泳迁移率分析核因子κB 与DNA 的结合活性。结果褪黑素能够显著减少ox-LDL所致的巨噬细胞TNF-α的释放，且p65／核因子κB的核移位减少，核因子κB 与DNA 的结合活性降低。结论褪黑素减少ox-LDL所致的巨噬细胞TNF-α的释放与其调节核因子κB的活性有关。 |
| 英文摘要: |
| ObjectiveTo explore the effect of melatonin on the release of tumor necrosis factor-α (TNF-α)and the activity of nuclear factor-κB(NF-κB)elicited by oxidized low-density lipoprotein(ox-LDL).MethodsRAW264.7 macrophage were treated with ox-LDL alone or accompanied with different concentrations of melatonin，the level of TNF-α in cell culture supernatants was measured by ELISA，the level of nuclear pool of p65/NF-κB protein was examined by Western blot for NF-κB nuclear translocation analysis，and the DNA binding activity of NF-κB was determined by EMSA.ResultsMelatonin inhibits the TNF-α release induced by ox-LDL，and melatonin also inhibits NF-κB translocation and DNA binding activity.ConclusionsThe effect of melatonin blocked the release of the TNF-α was related to inhibiting the NF-κB pathway. |
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