| 曾颖，冯惊涛，孙玉慧，孙琳，曾德星，易光辉.SR-BI过表达对J774细胞胆固醇转运的影响.[J].中南医学科学杂志.,2011,39(1):10-13. |
| SR-BI过表达对J774细胞胆固醇转运的影响 |
| Effect of Overexpression of SR-BI on Cholesterol Trafficking in J774 Cells |
| 投稿时间：2010-12-17 |
| DOI： |
| 中文关键词: SR-BI 单核/巨噬细胞 转染 过表达 胆固醇转运 |
| 英文关键词:SR-BI monocyte/macrophage transfection overexpression cholesterol trafficking |
| 基金项目:湖南省教育厅重点基金资助项目(09A078). |
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| 中文摘要: |
| 目的用构建的人SR-BI（hSR-BI）真核表达载体转染J774细胞，观察细胞瞬时过表达SR-BI对J774细胞胆固醇转运功能的影响。方法用hSR-BI cDNA转染法转染J774细胞，用RT-PCR和Western blot检测各组细胞的SR-BI表达，优化转染条件；用液体闪烁计数法检测流入和流出细胞的［3H］胆固醇量，分析过表达SR-BIJ774细胞的胆固醇转运功能。结果与对照组和空载体转染的J774细胞相比，pcDNA3.1(-)+hSR-BI转染的J774细胞SR-BI表达升高；与空载体转染的J774细胞相比，pcDNA3.1(-)+hSR-BI转染的J774细胞的胆固醇双向转运功能增强。结论用hSR-BI真核表达载体pcDNA3.1(-)+hSR-BI成功转染了J774细胞，使细胞过表达SR-BI，过表达的hSR-BI具有促进胆固醇转运的作用。 |
| 英文摘要: |
| ObjectiveTo investigate the effect of overexpression of SR-BI on the cholesterol trafficking in J774 cells.MethodsJ774 cells were transiently transfected by cloned hSR-BI to express hSR-BI,an 82 kDa protein.The expression of SR-BI were detected by RT-PCR and Western blot.The influx and efflux of［3H］ cholesterol from cells were determined by liquid scintillation counting.ResultsThe human scavenger receptor class B type I (hSR-BI) cDNA was successfully transfected into J774 cells.The expression level of SR-BI was elevated in J774 cells transfected with pcDNA3.1(-)+hSR-BI compared to control and vector (P<0.05).The function of cholesterol trafficking in J774 cells transfected with pcDNA3.1(-)+hSR-BI was reinforced compared to control and vector.ConclusionOverexpression of SR-BI can promote cholesterol trafficking in J774 cells. |
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