| 尹蔚兰，何剑琴，唐国华，张恺芳，唐小卿.硫化氢对MPP+诱导 PC12 细胞氧化应激损伤的保护作用.[J].中南医学科学杂志.,2011,39(1):7-9. |
| 硫化氢对MPP+诱导 PC12 细胞氧化应激损伤的保护作用 |
| Protective Effect of Hydrogen Sulfide on MPP+-Induced Oxidative Stress Damage in PC12 Cells |
| 投稿时间：2010-09-01 |
| DOI： |
| 中文关键词: 硫化氢 MPP+ PC12 细胞 氧化应激 |
| 英文关键词:hydrogen sulfide PC12 cells MPP+ oxidative stress |
| 基金项目:国家自然科学基金资助项目(No.30770740)；湖南省衡阳市科技局资助项目（2009HKJ12）. |
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| 中文摘要: |
| 目的探讨硫化氢对MPP+诱导PC12细胞氧化应激损伤的保护作用。方法以MPP+损伤 PC12 细胞作为帕金森病的细胞模型，甲氮甲唑蓝(MTT)法检测细胞存活率；丙酮酸二硝基苯腙比色法检测细胞上清液乳酸脱氢酶(LDH)漏出量；硫代巴比妥法检测细胞上清液中丙二醛(MDA)浓度；双氢罗丹明123 染色FCM检测细胞内活性氧水平变化；应用硫化氢钠(sodium hydrosulfide，NaHS)作为H2S的供体。结果200 μmol/L 和400 μmol/L 硫氢化钠呈浓度依赖性阻断MPP+引起PC12 细胞存活率的降低；400 μmol/L硫氢化钠能明显抑制400 μmol/L MPP+诱导的PC12细胞LDH的漏出，以及抑制MDA和活性氧的产生。结论硫化氢对MPP+诱导 PC12 细胞的氧化应激损伤具有保护作用。 |
| 英文摘要: |
| Objective To study the protective effect of hydrogen sulfide(H2S)on rat PC12 cells oxidative stress damage induced by 1-Methyl 4-phenylpyridiniumion(MPP+)in vitro.MethodsUsing MPP+ induced PC12 cells damage as the cell model of Parkinsons disease;The viability of PC12 cells was observed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay;The transudation content of lactate dehydregenase(LDH)was measured by colorimetrically;The level of malondialde-hyde(MDA)in supernatant-conditioned media was monitored by thiobituric acid reaction(TBA).The level of reactive oxygen species(ROS)in PC12 cells was tested by dihydrohodamine 123 stain FCM;Sodium hydrosulfide(NaHS)was used as H2S donor.ResultsNaHS(200 and 400 μmol/L)dose-dependently blocked the inhibition of cell viability induced by MPP+;400 μmol NaHS significantly inhibited the loss of LDH，the overproduction of MDA and ROS in PC12.Conclusion H2S has the protective effect on PC12 cells oxidative stress damage induced by MPP+. |
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