| 彭忠田,谭德明,黄顺玲,朱平安,刘菲.乙型肝炎免疫球蛋白聚氰基丙烯酸正丁酯纳米粒肝靶向性的初步研究.[J].中南医学科学杂志.,2010,(2):. |
| 乙型肝炎免疫球蛋白聚氰基丙烯酸正丁酯纳米粒肝靶向性的初步研究 |
| Preliminary Study of Liver-targeting of Hepatitis B Immunoglobulin Polybutylcynaoacrylate Nanoparticles |
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| DOI： |
| 中文关键词: 乙型肝炎免疫球蛋白 乙型肝炎免疫球蛋白聚氰基丙烯酸正丁酯纳米粒 肝靶向 免疫组织化学 |
| 英文关键词:hepatitis B immunoglobulin HBIG-PBCA-NP liver-targeting immunohistochemistry |
| 基金项目:湖南省长沙市科学技术局基金资助(K051155-72) |
| 彭忠田 谭德明 黄顺玲 朱平安 刘菲 |
| 南华大学第一附属医院肝病研究中心;中南大学湘雅医院; |
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| 中文摘要: |
| 目的初步探讨乙型肝炎免疫球蛋白聚氰基丙烯酸正丁酯纳米粒(HBIG-PBCA-NP)的肝靶向性。方法HBIG-PBCA-NP与HepG2.2.15细胞及QSG7701细胞共孵育,透射电镜观察细胞内的纳米粒;以HBIG-PBCA-NP、PBCA-NP或HBIG尾静脉注射昆明小鼠,透射电镜观察肝细胞内的纳米粒,免疫组织化学检测肝组织HBIG的含量。结果透射电镜照片显示HepG2.2.15细胞和QSG7701细胞的胞核和胞浆内可见纳米粒,昆明种小鼠肝组织中可见大量的纳米粒;肝组织HBIG免疫组织化学结果表明相同浓度的HBIG-PBCA-NP组比HBIG组显色更明显(P<0.01)。结论HBIG-PBCA-NP较HBIG肝靶向性更好。 |
| 英文摘要: |
| Objective To evaluate the liver-targeting of HBIG-PBCA-NP in vitro and in vivo. Methods QSG7701 cells and HepG2.2.15 cells were cultured with DMEM and HBIG-PBCA-NP,the cells was collected for detection of the nanoparticle by transmission electron microscope(TEM);HBIG-PBCA-NP,PBCA-NP and HBIG were administrated to the mice by the tail intravenous injection,then the mice were killed and the liver tissiue were collected for detecting the nanoparticle by TEM and HBIG by immunohistochemistry. Results After inocu... |
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