| 楚艳娥,朱建思,魏建国,周建国.siRNA干扰Msi1对人胃癌SGC-7901细胞的影响.[J].中南医学科学杂志.,2010,(2):. |
| siRNA干扰Msi1对人胃癌SGC-7901细胞的影响 |
| Downregulation of Msi1 by RNAi in Effect of SGC-7901 Cell Line |
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| DOI： |
| 中文关键词: Msi1 RNAi 胃癌 SGC-7901细胞 |
| 英文关键词:msi1 RNA gastric carcinoma |
| 基金项目: |
| 楚艳娥 朱建思 魏建国 周建国 |
| 南华大学肿瘤研究所; |
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| 中文摘要: |
| 目的探讨siRNA沉默Msi1基因表达对人胃癌SGC-7901细胞增殖、凋亡、侵袭与转移的影响。方法针对Msi1 mRNA序列设计合成siRNA,转染SGC-7901细胞。RT-PCR法检测Msi1基因表达;Westernblot检测survivin、caspase3及MMP-9表达;MTT法检测SGC-7901细胞增殖;流式细胞术检测细胞凋亡情况。结果Msi1 siRNA能有效抑制胃癌SGC-7901细胞中Msi1的表达,与空白对照组比较,Msi1 siRNA组SGC-7901细胞生长、增殖速度减缓(P<0.05);survivin、MMP9蛋白表达水平显著下调(P<0.05),而caspase3蛋白表达水平上调(P<0.05);Msi1 siRNA组凋亡率增高。结论沉默Msi1对人胃癌SGC-7901细胞增殖、凋亡、侵袭与转移有负性调节作用。 |
| 英文摘要: |
| Objective To investigate effect of silencing Msi1 gene with siRNA on cell proliferation,apoptosis,invasion and metastasis of SGC-7901 cell line. Methods Msi1-specific siRNA was designed and synthesized.Small interfering RNA was transfected into SGC-7901 cells to knockdown the Msi1 expression.mRNA levels of Msi1 was detected by RT-PCR,protein levels of surviving,caspase3 and MMP9 was detected by Western blot.The cell growth inhibition was determined by MTT.The effect of Msi1 siRNA on cell apoptosis was assay... |
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