| 廖前进,苏坚,何洁,宋颖,唐海林,苏琦.DADS对人结肠癌SW480细胞系增殖的影响.[J].中南医学科学杂志.,2010,(1):. |
| DADS对人结肠癌SW480细胞系增殖的影响 |
| Proliferation Effect in SW480 Cell Line With DADS |
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| DOI： |
| 中文关键词: 二烯丙基二硫 结肠癌 抑制增殖 MTT法 PCNA |
| 英文关键词:diallyl disulfide colon Cancer proliferation inhibition MTT PCNA |
| 基金项目:湖南省教育厅课题(No.05C455,No.06C694) |
| 廖前进 苏坚 何洁 宋颖 唐海林 苏琦 |
| 南华大学肿瘤研究所; |
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| 中文摘要: |
| 目的观察二烯丙基二硫(DADS)对人结肠癌SW 480细胞增殖的抑制作用。方法实验分DADS处理组和未处理组。MTT法、集落形成实验检测细胞增殖活性,光镜和电镜观察细胞形态学变化,流式细胞术检测周期分布,免疫细胞化学检测增殖细胞核(PCNA)表达改变。结果MTT和集落形成实验显示DADS作用SW 480细胞后,细胞生长抑制率呈浓度、时间依赖性增加,而集落形成率显著降低;组织学显示处理组细胞异型性降低,核质比下降,细胞器丰富;流式细胞仪分析显示DADS明显将细胞周期阻滞于G2/M期,且呈剂量依赖关系;免疫细胞化学显示处理组较未处理组细胞PCNA蛋白表达明显减弱(P<0.05)。结论DADS可体外抑制人结肠癌SW 480细胞增殖,使癌细胞阻滞于G2/M期,可能与下调PCNA蛋白的表达有关。 |
| 英文摘要: |
| Objective To investigate the inhibitory effect of diallyl disulfide(DADS) on human colon cancer cell line SW480 in vitro. Methods The experiment was divided into two groups: treated group with DADS and untreated group.SW480 cells proliferation was measured by MTT assay and colony forming.The morphology of tumor was observed under optical microscope and electron microscope.Cell cycle distribution was analyzed by flow cytometry(FCM).The expression of proliferating cell nuclear antigen(PCNA) was detected by im... |
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