| 杨阳,马云,符向辉,何淑雅.FXR1真核表达载体的构建及鉴定.[J].中南医学科学杂志.,2010,(1):. |
| FXR1真核表达载体的构建及鉴定 |
| Construction and Identification of Eukaryotic Expression Vector of FXR1 |
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| DOI： |
| 中文关键词: 脆性X综合征 FXR1基因 免疫共沉淀 |
| 英文关键词:fragile X syndrome FXR1 gene Co-immunoprecipitation |
| 基金项目:湖南省自然科学基金(07JJ3030);;湖南省卫生厅(B2006-099)资助项目 |
| 杨阳 马云 符向辉 何淑雅 |
| 南华大学生物化学与分子生物学教研室; |
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| 中文摘要: |
| 目的构建携带人脆性X相关基因1(FXR1)的真核表达载体pCMV-HA,为研究FXR1P互作蛋白奠定基础。方法以pYESTrp3-FXR1为模板进行聚合酶链反应(PCR)特异扩增FXR1基因片断,将扩增片段经EcoR I和XhoI双酶切后克隆到pCMV-HA载体,酶切鉴定阳性克隆并测序鉴定。结果成功构建了pC-MV-HA/FXR1真核表达载体。结论pCMV-HA/FXR1真核表达载体的构建为进一步在细胞内鉴定FXR1互作蛋白奠定了基础,从而对研究FXR1在脆性X综合征中的作用机理具有深远意义。 |
| 英文摘要: |
| Objective To clone human fragile-X-related gene 1(FXR1)and construct its eukaryotic expression vector in order to study the interactive protein with FXR1P. Methods The FXR1 gene was amplified from pYESTrp3/FXR1 plasmid,digested with EcoRI and XhoI and then cloned into eukaryotic expression vector pCMV-HA.The recombinant plasmid of pCMV-HA/FXR1 was verified by enzyme digestion and sequence analysis. Results The construction of eukaryotic expression vector pCMV-HA/FXR1 was successful and the resulted sequence... |
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