| 凌宏艳,庹勤慧,胡弼,欧和生,刘刚,奉水东,朱炳阳,何剑琴,廖端芳.3T3-L1前脂肪细胞分化过程中miRNAs表达谱的变化.[J].中南医学科学杂志.,2010,(1):. |
| 3T3-L1前脂肪细胞分化过程中miRNAs表达谱的变化 |
| Differential Expression Profiles of miRNAs During 3T3-L1 Pre-adipocyte Differentiation |
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| DOI： |
| 中文关键词: miRNAs表达谱 3T3-L1前脂肪细胞 脂肪细胞分化 |
| 英文关键词:miRNA expression profile 3T3-L1 preadipocytes adipocyte differentiation |
| 基金项目:国家自然科学基金资助(30770868,30600249) |
| 凌宏艳 庹勤慧 胡弼 欧和生 刘刚 奉水东 朱炳阳 何剑琴 廖端芳 |
| 南华大学生理学教研室;南华大学药理学教研室;南华大学流行病学教研室; |
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| 中文摘要: |
| 目的探讨3T3-L1前脂肪细胞分化过程中微小RNA(miRNAs)表达谱的变化。方法3T3-Ll前脂肪细胞培养和诱导分化,通过形态学观察和RT-PCR方法,判断3T3-Ll前脂肪细胞是否分化成脂肪细胞。运用miRNAs芯片技术检测3T3-Ll前脂肪细胞和脂肪细胞中差异表达miRNAs。结果3T3-L1前脂肪细胞呈现成纤维状,诱导分化的第9天,细胞变成球形,内含许多脂滴;过氧化物酶体增殖物激活受体r2(PPARr2)和脂肪性脂肪酸结合蛋白(aP2)的表达显著增加。3T3-L1前脂肪细胞分化成脂肪细胞上调的miRNAs有26个,下调的miRNAs有2个。结论3T3-L1前脂肪细胞分化过程中存在miRNAs表达谱的变化。 |
| 英文摘要: |
| Objective To investigate the differentially expressed miRNAs in the procession of 3T3-L1 preadipocytes differentiation. Methods 3T3-L1 preadipocytes were cultured and induced to differentiate,the extent of adipocyte differentiation were assessed by microscopic observation and reverse trancription PCR(RT-PCR) methods.MicroRNA microarray was used to investigate the differentially expressed miRNAs in 3T3-L1 adipocytes and adipocytes. Results 3T3-L1 pre-adipocytes exhibied a fibroblast-like morphology which was... |
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