| 宁玲,吕昌银,陈云生,范翔,贺元文.PAN-Rh6G能量转移荧光猝灭法测定痕量镍(Ⅱ).[J].中南医学科学杂志.,2008,(3):. |
| PAN-Rh6G能量转移荧光猝灭法测定痕量镍(Ⅱ) |
| Determination of Trace Nickel Based on Fluorescence Quenching the Energy Transfer System With Rhodanmine 6G-Complex of PAN-Ni~(2+) |
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| DOI： |
| 中文关键词: 镍 能量转移 荧光猝灭 罗丹明6G |
| 英文关键词:PAN |
| 基金项目: |
| 宁玲 吕昌银 陈云生 范翔 贺元文 |
| 南华大学公共卫生学院卫生化学检测学教研室,湖南衡阳421001 |
| 摘要点击次数: 743 |
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| 中文摘要: |
| 目的 根据能量转移荧光猝灭程度,建立一种荧光猝灭法测定环境水样中痕量镍的新方法.方法 在无水乙醇介质中,Ni2 与1-(2-吡啶偶氮)-2-萘酚(PAN)形成PAN-Ni2 配合物,其吸收光谱与罗丹明6G(Rh6G)的发射光谱有效重叠,发生能量转移,使Rh6G荧光猝灭,从而建立了痕量镍的荧光猝灭测定新方法.结果 在优化实验条件下,在24.3～640 ng/mL浓度范围内,Rh6G荧光猝灭程度与Ni2 质量浓度(pNi2 )呈现良好的线性关系(r=0.9996).方法 的检出限为7.3 ng/mL;相对标准偏差为0.73%～2.03%.结论 此方法灵敏、快速、简便,用于环境水样中痕量镍的测定,结果满意. |
| 英文摘要: |
| Objective To set up a novel fluorescence quenching method for the determination of trace Nickel based on the energy transfer.Methods In dehydrated alcohol,the fluorescence intensity of Rh6G was diminished greatly due to the energy transfer from Rh6G to complex PAN-Ni2+.A novel fluorescence quenching method for the determination of trace Nickel had been developed with the Rh6G-PANNi2+ energy transfer system.Results Under the optimum experimented conditions,the linear range of calibration curves for the deter... |
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