| 崔亚兰,曹诗运.雷帕霉素对HL-60细胞增殖和凋亡的影响.[J].中南医学科学杂志.,2008,(3):. |
| 雷帕霉素对HL-60细胞增殖和凋亡的影响 |
| Effect of Rapamycin on the Proliferation and Apoptosis of Human Leukemia Cell Line HL-60 |
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| DOI： |
| 中文关键词: 白血病 雷帕霉素 增殖 凋亡 |
| 英文关键词:leukemia Rapamycin proliferation apoptosis |
| 基金项目: |
| 崔亚兰 曹诗运 |
| 南华大学生理教研室,湖南衡阳421001 |
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| 中文摘要: |
| 目的 研究雷帕霉素作用于HL-60细胞后,对细胞增殖、细胞周期及细胞凋亡率的影响.方法 通过细胞生长曲线、MTT检测细胞增殖情况;免疫荧光染色、流式细胞仪检测细胞周期和凋亡率.结果 雷帕霉素组与空白对照组细胞生长抑制率比较,差异有显著性(P<0.05);细胞培养72 h后,雷帕霉素组与空白对照组细胞凋亡率比较,差异有显著性(P<0.05).结论 雷帕霉素能抑制HL-60细胞增殖及促进其凋亡. |
| 英文摘要: |
| Objective To investigate the effct of Rapamycin on the proliferation,cell cycle and apoptosis of human leukemia cell line HL-60.Methods HL-60 cells were treated with various doses of rapamycin,the proliferation of HL-60 cells was detected by growth curve and MTT method.Immunofluorescent staining and flow cytometer was used for cell cycle and appoptosis detection. Results Compaired with rapamycin and control group,there is a significant difference of cell growth inhibition rate between control group and rapa... |
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