| 全细云,朱建思,张亮,刘慧敏,周建国.人胃癌高低侵袭转移潜能细胞系对化疗药物敏感性的比较研究.[J].中南医学科学杂志.,2008,(3):. |
| 人胃癌高低侵袭转移潜能细胞系对化疗药物敏感性的比较研究 |
| A Comparative Study of Chemotherapy Sensitivity in Human Gastric Carcinoma Cell Lines With High-and Low-Potential of Invasiveness and Metastasis |
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| DOI： |
| 中文关键词: 胃癌 侵袭转移 化疗敏感性 |
| 英文关键词:Gastric carcinoma Invasion and metastasis Chemotherapy sensitivity |
| 基金项目: |
| 全细云 朱建思 张亮 刘慧敏 周建国 |
| 南华大学肿瘤研究所,湖南衡阳421001 |
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| 中文摘要: |
| 目的 探讨肿瘤细胞侵袭转移能力的改变对肿瘤细胞化疗敏感性的影响.方法 Westem blotting法比较筛选出的人胃癌细胞MKN-28S(亚系)与MKN-28(母系)中E-Cadherin、MMP-2蛋白表达;transwell体外侵袭实验比较两系侵袭潜能.采用MTT法比较两系对阿霉素(ADM)、丝裂霉素(MMC)、5-氟尿嘧啶(5-Fu)、紫杉醇(PTX)4种化疗药物的敏感性.结果 筛选出亚系的E-Cadherin 蛋白表达与母系相比显著减弱、而MMP-2蛋白表达则显著增强;显微镜下细胞计数示亚系侵袭至基质胶膜背面的数量较母系明显增多(P<0.05);MTY示两系对5-Fu、ADM、MMC的敏感性差异有显著性(P<0.05),而两系对PTX的敏感性差异无显著性(P>0.05).结论 肿瘤细胞侵袭转移能力的改变可以影响肿瘤细胞对化疗药物的敏感性,同一遗传背景下的高侵袭转移亚系较母系耐药性更强. |
| 英文摘要: |
| Objective To explore effects of alteration in tumor cells' invasiveness and metastasis on its chemotherapeutic sensitivity. Methods Protein expression level of E-Cadherin and MMP-2 in MKN-28S and MKN-28 cell lines was assessed by Western blot.The invasiveness was assessed by penetration through a matrigel-coated filter.MTT assays were used to detect sensitivity of two cell lines by ADM,MMC,5-Fu,and PTX treatment. Result Protein expression level of E-cadherin was significantly decreased and that of MMP-2 sig... |
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