| 邓宇傲,赵方,李华珍,谢宛玉.2种人卵巢癌裸鼠皮下移植瘤模型制备方法的比较.[J].中南医学科学杂志.,2008,(2):. |
| 2种人卵巢癌裸鼠皮下移植瘤模型制备方法的比较 |
| Comparison on Establishing Subcutaneous Tumor Bearing Nude Mice Model With Human Ovarian Adenocarcinoma Cell Line in Two Different Methods |
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| DOI： |
| 中文关键词: 卵巢癌COC1细胞株 荷瘤模型 裸小鼠 皮下移植瘤 |
| 英文关键词:Ovarian adenocarcinoma COC1 Cell lines Tumor-bearing model Nude mice Subcutaneous transplanted tumor |
| 基金项目: |
| 邓宇傲 赵方 李华珍 谢宛玉 |
| [1]南华大学第一附属医院妇产科,湖南衡阳421001 [2]南华大学生命科学与技术学院,湖南衡阳421001 |
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| 中文摘要: |
| 目的 比较直接接种人卵巢癌COC1细胞和COC1细胞经体内传代后接种Balb/c-nu裸鼠皮下移植瘤模型制备方法的成瘤率.方法 雌性突变系Balb/c-nu小鼠40只,分为对照组及实验组,每组20只.COC1细胞注射于裸鼠一侧背部皮下,对照组细胞培养后即接种,观察统计数据后处死,取瘤体制成单细胞悬液再接种于实验组裸鼠,比较两种不同方法接种的成瘤率,并观察成瘤时间、瘤重及肿瘤生长速度.结果 实验组成瘤率为70%,对照组仅为30%.结论 经过裸鼠体内传代后的COC1细胞用于制备人卵巢癌裸鼠皮下移植瘤模型具有较高的成瘤率. |
| 英文摘要: |
| Objective To establish the best way of tumor-bearing Balb/c-nu nude mice model with Epithelial varian adenocarcinoma cell line COC1.Methods Balb/c-nu nude mice were divided into experimental group(EG) and control group(CG);COC1(suspended cell)cells were injected into nude mice subcutaneous layers of back.In EG,COC1 cells were passaged in vivo of nude mice,but COC1 cells in CG were not passaged.The rate of tumor formation of different methods was compared and the time of tumor formation,the weight annd growt... |
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