| 羊明智,吴叶,章亚东,候树勋,贾连顺.钙调素及其依赖性激酶对即早基因c-fos表达的影响.[J].中南医学科学杂志.,2007,(4):529-532. |
| 钙调素及其依赖性激酶对即早基因c-fos表达的影响 |
| The Effect of the CAM and CAM-Dependent Kinase on the Expression of c-fos Gene in the Spinal Cord Matter |
| 投稿时间：2007-05-15 |
| DOI： |
| 中文关键词: 钙调素 钙调素蛋白激酶 离体脊髓组织培养 c-fos 钙调素 依赖性激酶 即早基因 表达变化 影响 Matter Spinal Cord Gene Expression Kinase 激活 复合物 钙离子 离子浓度 杂交信号 mRNA 对照组 钙浓度 结果 时间点 |
| 英文关键词:CAM CAM - PK spinal cord slice culture c - fos |
| 基金项目: |
| 羊明智 吴叶 章亚东 候树勋 贾连顺 |
| [1]南华大学第一附属医院脊柱外科,湖南衡阳421001 [2]北京304医院,湖南衡阳421001 [3]上海长征医院,湖南衡阳421001 |
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| 中文摘要: |
| 目的 探讨钙调素及其依赖性激酶对脊髓灰质c-fos基因表达的影响.方法 用原位杂交方法分别观察高钙环境下离体培养脊髓组织在应用钙调素及其依赖性激酶拮抗剂(三氟啦嗪及KN-62)环境下30 min至12 h不同时间点c-fos基因的表达变化.结果 高钙浓度组信号明显较对照组、三氟啦嗪组及KN-62组强.高钙浓度组c-fos mRNA杂交信号在4～6 h时间点达高峰.三氟啦嗪组及KN-62组杂交信号在6 h时间点较对照组增高,但比高钙离子浓度组弱.结论 钙离子介导c-fos表达可能是与钙调素形成复合物激活CAM-PK实现的. |
| 英文摘要: |
| Objective To study the effect of CAM and CAM - dependent kinase on the the expression of c - fos gene in the spinal cord matter. Methods After the cultured spinal cord slices in high calcium ions situation treated with CAM and CAM- dependent kinase at the time from 30 minuts to 12 hour ,the hybrid in situ was studied to reveal the expression of c - los gene. Results The strongest expression was found in the high calcium ions group, and the expression reached the maxium at 4 - 6 h after treatment. The enhanced expression happened in the TFP group and the KN - 62 group at the 6h after treatment. Conclusions The action of calcium ions inducing the expression of c - fos may be realized by compounding with CAM, then rousing CAM - PK. |
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