| 何洁,万炜,廖前进,谭晖,周秀田,黄卫国,谢海龙,苏琦.DADS诱导HL-60细胞分化中的信号传导相关蛋白.[J].中南医学科学杂志.,2007,(4):477-480. |
| DADS诱导HL-60细胞分化中的信号传导相关蛋白 |
| The Signal Transduction-Associated Protein in HL-60 Cell Induced by DADS |
| 投稿时间：2007-03-27 |
| DOI： |
| 中文关键词: 双向电泳 质谱分析 二烯丙基二硫 HL-60细胞 分化 DJ-1蛋白 DADS 细胞分化 信号传导通路 相关蛋白 Induced Cell Protein JAKs 活性 转录因子 STATs 蛋白抑制 PIAS 活化 蛋白合成 转录调节 功能 表达下调 差异蛋白质 比较 |
| 英文关键词:two- dimensional electrophoresis mass spectrometry diallyl disulfide HL - 60 differentiation DJ- 1 protein |
| 基金项目:湖南省卫生厅科研项目
,
湖南省教育厅资助项目 |
| 何洁 万炜 廖前进 谭晖 周秀田 黄卫国 谢海龙 苏琦 |
| [1]南华大学肿瘤研究所,湖南衡阳421001 [2]南华大学解剖学教研室,湖南衡阳421001 |
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| 中文摘要: |
| 目的 研究二烯丙基二硫(DADS)诱导人髓性白血病HL-60细胞分化过程中蛋白质组的差异表达.方法 运用蛋白质组学方法(双向凝胶电泳、质谱分析、生物信息学方法)分析鉴定DADS诱导HL-60细胞分化的差异表达蛋白质.结果 DADS作用HL-60细胞后,细胞形态发生明显变化,NBT还原能力明显增强.与未处理组比较,初步鉴定的差异蛋白质DJ-1蛋白在处理组中表达下调,其功能与转录调节有关.结论 DADS可能通过抑制DJ-1蛋白合成,活化PIAS蛋白抑制STATs转录因子的活性,进而抑制JAKs/STATs信号传导通路,诱导HL-60细胞分化. |
| 英文摘要: |
| Objective To study differential proteomic expression in the differentiation of HL-60 cells induced by diallyl disulfide(DADS).Methods Differential expression of cellular proteins in HL-60 cell induced by DADS were analyzed and identified via proteomic approach,two dimensional gel electrophoresis,mass spectroscopy and bioinformatics.Results The cytomorphosis of the DADS-treated cells was significantly different from controls.NBT reduction was increased in the DADS-treated cells.Among the identified differential expression protein,the expression of DJ-1 protein was decreased in DADS-treated group.Its function was associated with transcription.Conclusions Inhibition expression of DJ-1 protein was involved in HL-60 cells differentiation induced by DADS and might associate with inhibition of JAKs/STATs single pathways via activating PIAS protein. |
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