| 何湘珍,刘嘉毅,周小平.甘草次酸对晶状体上皮细胞增殖的抑制作用.[J].中南医学科学杂志.,2007,(2):181-185. |
| 甘草次酸对晶状体上皮细胞增殖的抑制作用 |
| Inhibitive Effect of GA on Rabbit Lens Epithelial Cells Proliferation |
| 投稿时间：2006-10-17 |
| DOI： |
| 中文关键词: 甘草次酸 晶状体上皮细胞 凋亡 TUNEL 流式细胞仪 甘草次酸 晶状体上皮细胞增殖 抑制作用 Proliferation Rabbit Lens Epithelial Cells 时间依赖性 剂量 诱导作用 细胞凋亡率 延长 结果 定量检测 flow 流式细胞仪 利用 凋亡细胞 鉴别 TUNEL terminal mediated |
| 英文关键词:glycyrrhetinic acid lens epithelial cells cell apoptosis TUNEL flow cytometer |
| 基金项目:湖南省社会科学基金 |
| 何湘珍 刘嘉毅 周小平 |
| 南华大学第二附属医院,湖南衡阳421001 |
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| 中文摘要: |
| 目的 研究甘草次酸(glycyrrhetinic acid,GA)对培养的兔晶状体上皮细胞(rabbit lens epithelial cells,RLECs)增殖的影响及其机理.方法 首先进行RLECs的体外培养,取第3代的RLECs分别加入不同浓度的GA和20 mg/L的MMC处理,观察细胞形态学变化、免疫细胞化学方法--脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(terminal -deoxynucleotidyl transferase mediated nick end labeling, TUNEL)进一步鉴别凋亡细胞,利用流式细胞仪(flow cytometer,FCM)对细胞凋亡率进行定量检测.结果 GA对体外培养的RLECs的增殖有显著的影响, RLECs的凋亡率随着GA的浓度增加和时间的延长而增加.结论 GA可以引起体外培养的RLECs凋亡,且对RLECs凋亡诱导作用呈剂量和时间依赖性. |
| 英文摘要: |
| Objective To study the effect and mechanisms of glycyrrhetinic acid (GA) on the proliferation of rabbit lens epithelial cells(RLECs) in vitro and to provid the theory reference for developing the drug of preventioning and curing posterior capsular opacification(PCO).Methods RLECs were cultured in vitro first and GA of different concentrations and 20 mg/L MMC were added to the rabbit lens epithelial cells of third passage.We observed cells morphological changes with light microscope and identified the apoptosis with immunocytochemical method, terminal-deoxynucleotidyl transferase mediated nick end labeling(TUNEL)and used flow cytometry(FCM) as a quantitative method of evaluation of these effect.Results GA could significantly affect the proliferation of RLECs.The apoptosis rate of RLECs was increased with the increase of the GA concentrations and the longing of time.Conclusion GA can induce apoptosis of rabbit lens epithelial cells cultured in vitro.The apoptosis rate of RLECs was depended on the GA concentrations and the longing of time. |
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