| 谭滇湘,张朝晖,郭曲练,蔡宏伟.罗哌卡因对大鼠血淋巴细胞DNA的损伤作用.[J].中南医学科学杂志.,2007,(1):71-73. |
| 罗哌卡因对大鼠血淋巴细胞DNA的损伤作用 |
| The DNA Damages of Blood Lymphocyte Cell After Celiac Injection Ropivacaine in Wistar Rats |
| 投稿时间：2006-09-15 |
| DOI： |
| 中文关键词: 罗哌卡因 单细胞凝胶电泳 淋巴细胞 DNA损伤 罗哌卡因 大鼠 血淋巴细胞 损伤作用 Wistar Rats Ropivacaine Injection Cell Lymphocyte Blood 存在 高浓度 统计学意义 差异 尾长 彗星 结果 电泳检测 单细胞凝胶 动物 |
| 英文关键词:ropivavaine single cell gel electrophoresis lymphocyte DNA damages |
| 基金项目: |
| 谭滇湘 张朝晖 郭曲练 蔡宏伟 |
| [1]中南大学湘雅医院麻醉学系,湖南长沙410008 [2]南华大学公共卫生学院,湖南长沙410008 |
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| 中文摘要: |
| 目的 观察罗哌卡因腹腔注射后对大鼠血液淋巴细胞DNA的影响,为不同浓度罗哌卡因的应用提供安全性依据.方法 Wistar大鼠72只,随机分为四组,对照组腹腔注射生理盐水,实验组(T1,T2,T3)按体重0.1 mL/100 g分别腹腔注射0.5%、0.75%、1.0%罗哌卡因.各组分别在腹腔注射后6、12和24小时3个时点各取6只动物,采用单细胞凝胶电泳检测血液淋巴细胞DNA损伤.结果 各实验组血淋巴细胞的彗星率和尾长均高于对照组,其中 T3组各时点与对照组相比差异有统计学意义(P＜0.05);T2组与对照组相比,在24小时时差异有显著性(P＜0.05).结论 低浓度(0.5%,0.75%)罗哌卡因腹腔注射24小时内对大鼠血液淋巴细胞无明显影响;高浓度罗哌卡因(1.0%)腹腔注射24小时内对大鼠血液淋巴细胞可能存在损伤作用. |
| 英文摘要: |
| Objective To explore the lymphocyte DNA damages after celiac injection ropivacaine in wistar rats and provide the experimental data for the security assessment of ropivacaine application.Methods Seventy-two male wistar rats were randomly and averagely divided into 4 groups.The groups were randomized to receive normal saline,0.5%,0.75% or 1.0% respectively by the celiac injection 0.1 mL every 100 g body weight.Six rats in every group in 6 h,12 h and 24 h after the injection were killed,and every rat was phlebotomized blood approximately 0.5 mL blood(anticoagulant by heparitin).The alkaline single cell gel electrophoresis was used to determine DNA damages of blood lymphocyte.Results The comet tail ratio and tail length of lymphocyte in 1.0% concentration group at 6 h,12 h and 24 h were higher or longer than that of the control group at corresponding time point(P<0.05),while that of 0.75% ropivavaine group compared with the control was significantly different only in 24 h(P<0.05).The comet tail ratio and tail length of lymphocyte in 0.5% concentration group at 6 h,12 h and 24 h compared with the control had no significant difference(P>0.05).Conclusions 0.5% and 0.75% ropivavaine do not have obvious DNA damage effect on rats' lymphocyte.But 1% ropivavaine can induce DNA damages of rats lymphocyte,and it may have potential risk to human. |
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