| 李燕雄,陈忠东.宫颈癌SiHa细胞脂质体与电穿孔转染效果比较.[J].中南医学科学杂志.,2006,(4):526-529. |
| 宫颈癌SiHa细胞脂质体与电穿孔转染效果比较 |
| Comparing Lipofection With Electrofection in Cervical Cancer Cell Lines SiHa |
| 修订日期：2006-03-18 |
| DOI： |
| 中文关键词: 脂质体 电穿孔 基因转染 宫颈癌 |
| 英文关键词:FHIT |
| 基金项目: |
| 李燕雄 陈忠东 |
| 南华大学第一附属医院妇产科,湖南衡阳421001 |
| 摘要点击次数: 953 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的探讨脆性组氨酸三联体基因（fragile histidine triad gene，FHIT）转染宫颈癌SiHa细胞过程中脂质体与电穿孔两种转染方法的效果有无区别。方法分别用脂质体和电穿孔方法将真核表达质粒PRcCMV—FHIT及PRcCMV转染SiHa细胞，用带绿色荧光蛋白的PEGFP—C2空质粒检测瞬时转染效率。结果脂质体与电穿孔两种方法的瞬时转染效率无明显差别，但转染过程中两种方法转染的细胞形态有差别；在筛选稳定转染细胞株的过程中，电穿孔法转染的细胞更易存活。结论两种方法均适于瞬时转染，而电穿孔法可能更适于稳定转染。 |
| 英文摘要: |
| Objective To compare the effect of lipofection with the effect of electrofection in cervical cancer cell lines SiHa.Methods The plasmids PRcCMV-FHIT and PRcCMV were transfected into SiHa cells by lipofectamine 2000 and electrofection respectively.The effect of transfection is tested by blank plasmid PEGFP-C_2.Results The effect of transtient transfection of two methods is not different,but the cells morphism is different.The cells electrofected are apted to live well in stable transfection.Conclusions Both methods are effective in transtient transfection.Electrofection may be more effective in stable transfection. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|