| 巫智勇,熊平,封元清,周秀田,周君,唐群,罗国厂.兔急性肺损伤死后肝内血管内皮生长因子时序性变化的研究.[J].中南医学科学杂志.,2006,(4):498-502. |
| 兔急性肺损伤死后肝内血管内皮生长因子时序性变化的研究 |
| Estimation of Postmortem Interval(PMI) From Levels of Vascular Endothelial Growth Factor(VEGF) in Liver of Rabbits Executed With Acute Lung Injury(ALI) |
| 修订日期：2006-04-11 |
| DOI： |
| 中文关键词: 法医病理学 死亡经过时间 急性肺损伤 血管内皮生长因子 免疫组织化学染色 |
| 英文关键词:forensic pathology,postmortem interval,acute lung injury,vascular endothelial growth factor,immunohistochemistry, |
| 基金项目: |
| 巫智勇 熊平 封元清 周秀田 周君 唐群 罗国厂 |
| [1]南华大学基础医学院,湖南衡阳421001 [2]湘潭市中心医院肿瘤内科,湖南衡阳421001 |
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| 中文摘要: |
| 目的研究大潮气量机械通气致兔急性肺损伤（ALI）12h后处死，死后肝微血管内血管内皮生长因子（VEGF）的动态变化。方法机械通气（VT60mL／kg，R50次／min）致急性肺损伤后12h处死兔，应用免疫组织化学染色（SABC法）和图像分析系统，观测死后不同时间点肝内微血管内皮细胞内vEGF的动态变化。结果对照组死后即刻肝内微血管内皮细胞胞浆vEGF呈弱阳性染色，死后2h阳性染色程度有轻度增加，但以后随PMI的延长VEGF逐渐减弱，死后18h呈阴性；试验组死后肝内微血管的内皮细胞胞浆VEGF呈强阳性表达，但随着PMI延长逐渐减弱，在死亡后0-24h减弱幅度较小，但21-36h阳性染色程度下降较快，直至死后42h随肝内微血管结构崩解而完全消失。结论大潮气量机械通气致急性肺损伤12h处死后，肝微血管内VEGF的变化在一定的时间段内与死亡时间呈现相关关系。 |
| 英文摘要: |
| Objective To explore the kinetic change of vascular endothelial growth factor(VEGF) in capillary vessel of liver after rabbits with acute lung injury induced by high-VT mechanical ventilation(V_T 60 mL/kg)were execute.Methods Immunohistochemistry and image-analysis techniques were employed in surveying the mean gray scale value of VEGF at difference postmortem interval(PMI).Results Control case:Weakly positive staining of VEGF was detected in cytoplasm of vascular endothelial cell(VEC) instantly after death and increased slightly in 2 hours subsequently,then decreased with prolong of PMI,and get negative staining at 18 h postmortem;trial group:strong positive staining of VEGF was discovered in cytoplasm of VEC instantly after death,tapered with extension of PMI,and faded at 42 h postmortem with structure of capillary vessel demolishing.Conclusion Within 36 hours after death,the change of VEGF in capillary vessel of liver correlated with PMI after rabbits with acute lung injury by high-VT mechanical ventilation were execute. |
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